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首页> 外文会议>International IEEE/EMBS Conference on Neural Engineering >Microelectrode arrays studies of glutamate excitatory pathway in hippocampus CA3 by offside KCl and glutamate stimulating
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Microelectrode arrays studies of glutamate excitatory pathway in hippocampus CA3 by offside KCl and glutamate stimulating

机译：越位KCl和谷氨酸刺激对海马CA3谷氨酸兴奋通路的微电极阵列研究

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In our central nervous system (CNS), glutamate is an excitatory neurotransmitter that can be accumulated remarkably by brain tissue. As is noticeable from previous studies, glutamate is somehow closely related with most ways of normal and abnormal cerebral activities. It was significant to monitor glutamate concentration changes in vivo real time. Therefore, we developed an enzyme-based microelectrode array with high sensitivity (7.34pA/µM) and perfect linearity (R=0.999). Selectivity to normal neurotransmitters satisfied our in vivo demands. Ultimately, we applied the electrode into the brain specific tissue to study glutamate excitatory pathway in hippocampus CA3 by offside KCl and glutamate stimulating. The hippocampus CA3 glutamate was detected about 18.17µM and 13.19µM by twice KCl stimulating in the offside cortex, and 6.50µM, 4.55µM by twice glutamate stimulating. All peak current back to the base level later. The self-regulation ability reflects the glutamate excitatory pathway from hippocampus CA3 to cortex.
机译：在我们的中枢神经系统（CNS）中，谷氨酸是一种兴奋性神经递质，可以被脑组织显着地积累。从以前的研究中可以看出，谷氨酸在某种程度上与正常和异常大脑活动的大多数方式密切相关。在体内实时监测谷氨酸盐浓度的变化非常重要。因此，我们开发了具有高灵敏度（7.34pA / µM）和完美线性（R = 0.999）的基于酶的微电极阵列。对正常神经递质的选择性满足了我们体内的需求。最终，我们将电极应用到大脑特定组织中，通过越位KCl和谷氨酸刺激研究海马CA3中的谷氨酸兴奋性途径。通过在越位皮层中两次KCl刺激，检测到海马CA3谷氨酸约为18.17µM和13.19µM，通过两次谷氨酸刺激可检测到6.50µM，4.55µM。以后所有峰值电流都回到基极水平。自我调节能力反映了谷氨酸从海马CA3到皮层的兴奋性途径。

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