首页> 外文会议>International biohydrometallurgy symposium >Metagenome-Derived Draft Genome Sequence of Acidithiobacillus ferrooxidans RV1 from an Abandoned Gold Tailing in Neuquen, Argentina
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Metagenome-Derived Draft Genome Sequence of Acidithiobacillus ferrooxidans RV1 from an Abandoned Gold Tailing in Neuquen, Argentina

机译:来自阿根廷内乌肯市废弃金尾矿的酸性氧化硫硫杆菌RV1的基因组衍生草图基因组序列。

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In this work we report the metagenome-derived draft genomic sequence of an enrichment culture dominated by A. ferrooxidans obtained from an airlift bioreactor inoculated with the microbial consortium recovered from the "Relave Viejo" tailing. The genome of this culture was assembled de-novo and by reference, generating a consensus assembly of 3.0 Mb. On the basis of 16S rRNA (100 % identity), average nucleotide identity analysis (99.33% identity) and in silico DNA-DNA hybridization against A. ferrooxidans ATCC 23270~T (97.9%), the recovered genome is confirmed to pertain to A. ferrooxidans species. Comparative genomics results are presented to uncover the genetic traits of the variant surviving lime treatment and to further explore the genomic diversity of these model iron oxidizing species.
机译:在这项工作中,我们报告了富氧培养物的基因组学衍生基因组序列草案,其中富氧培养物主要来自于从空运生物反应器中获得的接种了从“ Relave Viejo”尾矿中回收的微生物菌落的空运生物反应器。该培养物的基因组从头开始组装,并作为参考,产生了3.0 Mb的共有组装。根据16S rRNA(100%同一性),平均核苷酸同一性分析(99.33%同一性)以及抗氧化铁拟南芥ATCC 23270〜T(97.9%)的计算机DNA-DNA杂交,确认回收的基因组与A相关氧化铁物种。提出了比较基因组学结果,以揭示幸存的石灰处理变异体的遗传特征,并进一步探索这些模型铁氧化物种的基因组多样性。

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