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首页> 外文会议>Conference on optical methods for tumor treatment and detection: mechanisms and techniques in photodynamic therapy >Photodynamic inactivation of Staphylococcus aureus and Escherichia coli using a new bacteriochlorin as photosensitizer
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Photodynamic inactivation of Staphylococcus aureus and Escherichia coli using a new bacteriochlorin as photosensitizer

机译：使用新型细菌绿素作为光敏剂的金黄色葡萄球菌和大肠杆菌的光动力学灭活

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In this study, we used bacteriochlorin as a photosensitizer, characterized by their low toxicity in the absence of light, presenting absorption around 780 nm, with the objective of evaluating their photodynamic inactivation potential on Staphylococcus aureus and Escherichia coli. Bacteriochlorins were synthesized from the extraction of bacteriochlorophylls from non-sulfurous purple bacteria and were then converted to bacteriochlorins. 5. aureus and E. coli microorganisms were used in the planktonic and biofilm forms. For the formation of biofilms on glass coverslips, suspensions of the microorganisms at the concentration of 10~6 CFU/mL were inoculated into each well of a microplate. There was an exchange of culture medium (Tryptic Soy Broth - TSB) every 24 hours for 7 days, pre-washing the coverslips with a phosphate-buffered saline (PBS), to ensure that only adhered microorganisms were grown and then incubated at (36 ± 1)°C between the middle exchanges. After 7 days of induction, the biofilm was mature, like those normally found in nature, and then it was applied different treatments (light doses associated with FS concentrations). At the end of the treatment, the coverslips underwent an ultrasonic disintegration, and the quantitative evaluation of viable cells was performed by plate counting using the plate method in Tryptic Soy Agar (TSA), incubating at (36 ± 1)°C for 24 hours. The results showed that the PDI for E. coli was not successful even when it was more susceptible to the planktonic form, whereas for 5. aureus the results showed a reduction in cell viability 6 logs for the planktonic forms, but lower to 1 log in biofilms. Therefore, novel studies using bacteriochlorins and surfactants will be performed to verify the potential of this alternative treatment method.
机译：在这项研究中，我们使用细菌氯霉素作为光敏剂，其特征是在无光下毒性低，在780 nm处有吸收，目的是评估其对金黄色葡萄球菌和大肠杆菌的光动力失活潜力。从非含硫紫色细菌中提取细菌绿素可合成细菌绿素，然后将其转化为细菌绿素。 5.以浮游生物和生物膜形式使用金黄色和大肠杆菌微生物。为了在玻璃盖玻片上形成生物膜，将浓度为10〜6 CFU / mL的微生物悬浮液接种到微孔板的每个孔中。每24小时更换一次培养基（Tryptic Soy Broth-TSB），共7天，用磷酸盐缓冲液（PBS）清洗盖玻片，以确保只有粘附的微生物才能生长，然后在（36）下孵育中间交换之间为±1）°C。诱导7天后，生物膜已经成熟，就像自然界中正常发现的那样，然后对其进行了不同的处理（与FS浓度相关的轻剂量）。处理结束后，将盖玻片进行超声崩解，并在胰蛋白酶大豆琼脂（TSA）中使用平板方法通过平板计数对活细胞进行定量评估，并在（36±1）°C下孵育24小时。结果表明，即使对浮游生物更敏感，大肠杆菌的PDI也不成功，而对于5.金黄色葡萄球菌，结果表明浮游生物的细胞活力降低了6 log，而在浮游生物中则降低了1 log。生物膜。因此，将进行使用细菌绿素和表面活性剂的新研究，以验证这种替代治疗方法的潜力。

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