首页> 外文会议>Conference on single-use technologies II: bridging polymer science to biotechnology applications >APPLY ADSORPTION TECHNOLOGY TO SOLVE THE UV SENSOR INSTABILITY OF DYNAMIC CONTROL ON PERIODIC COUNTER CURRENT PURIFICATION SYSTEM
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APPLY ADSORPTION TECHNOLOGY TO SOLVE THE UV SENSOR INSTABILITY OF DYNAMIC CONTROL ON PERIODIC COUNTER CURRENT PURIFICATION SYSTEM

机译:应用吸附技术解决周期计数器电流净化系统动态控制中的紫外线传感器不稳定性

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Cell culture media are a source of major nutrients that provide cell growth and synthesis of proteins. Researchers and suppliers use combine media components test continually in an attempt to find more suitable cell culture media for use in continuous cell culture processes. Commercial media components are a black box for downstream purification researchers who cannot predict the effects of their constituents on resin affinity, absorbability, even for interference with UV instrument. To more effectively utilize in dynamic continuous purification system, the loading product percentage of resin absorbability is typically controlled at 40-60% maximum binding capacity. It is often five times more than traditional batch purification. Therefore, the swing of UV percentage caused by color material in the bulk harvest is the key process parameter in the periodic counter current purification system. We compared different commercial quaternary ammonium adsorbent matrix (such as Stabilized Regenerated Cellulose, Agarose and Styrene Divinyl Benzene Copolymer) to observe their effects on the swing of the UV amplitude. The results show that agarose group has better de-coloration efficacy than the other two commercial quaternary ammonium groups.
机译:细胞培养基是提供细胞生长和蛋白质合成的主要营养物质的来源。研究人员和供应商不断地使用组合培养基成分进行测试,以试图找到更适合用于连续细胞培养过程的细胞培养基。对于下游的纯化研究人员来说,商业介质成分是一个黑匣子,他们无法预测其成分对树脂亲和力,吸收性的影响,即使是对UV仪器的干扰也是如此。为了更有效地用于动态连续纯化系统中,通常将树脂吸收性的负载产物百分比控制在最大结合能力的40-60%。它通常是传统批量纯化的五倍。因此,大批量收获中由有色材料引起的UV百分比波动是周期性逆流净化系统中的关键工艺参数。我们比较了不同的商业季铵吸附剂基质(例如稳定的再生纤维素,琼脂糖和苯乙烯二乙烯基苯共聚物),以观察它们对UV振幅摆动的影响。结果表明,琼脂糖基团比其他两个商业季铵盐基团具有更好的脱色效果。

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