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首页> 外文会议>Conference on single-use technologies II: bridging polymer science to biotechnology applications >A VIRUS HARVEST UNIT (VHU) FOR THE CONTINUOUS HARVESTING OF LENTIVIRUS FROM SUSPENSION CELL CULTURES
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A VIRUS HARVEST UNIT (VHU) FOR THE CONTINUOUS HARVESTING OF LENTIVIRUS FROM SUSPENSION CELL CULTURES

机译：一种病毒收获单位（VHU），用于悬浮细胞培养物的连续收获慢病毒

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For virus particles produced by cell culture systems, free intact virus particles as well as virus particles associated with host cell membranes or other viral particles and damaged virus fragments will be present. To reduce the amount of host cell fragments and/or virus aggregates it is critically important to reduce the shear stress applied to the cell culture during the harvesting step as much as possible. This is mainly accomplished by utilizing a low-shear Virus Harvest Unit (VHU?) with large pore size membranes (5-15mM) and by utilizing a low-shear pump. As shown in Table Ⅰ, the VHU?, is able to clarify the broth containing the viral vectors from less than 500 Nephelometric Turbidity Units (NTU) down to less than 16 NTU. In contrast the turbidity of a broth harvested by centrifugation from a batch is around 50-90 NTU and the turbidity of the harvest stream from an acoustic filter (Biosep) is 33 NTU. These results suggest it may be possible to eliminate the secondary clarification steps when using the VHU unit as the virus harvesting process mode.
机译：对于细胞培养系统产生的病毒颗粒，存在与宿主细胞膜或其他病毒颗粒相关的游离完整的病毒颗粒以及病毒颗粒。为了减少宿主细胞片段和/或病毒聚集体的量，重要的是在尽可能地在收获步骤中减少施加到细胞培养的剪切应力是至关重要的。这主要是通过利用具有大孔径膜（5-15mm）的低剪切病毒收获单元（VHU？）来实现，并通过利用低剪切泵。如表Ⅰ所示，VHU？，能够将含有病毒载体的肉汤从小于500个浊度浊度单元（NTU）降至小于16 NTU。相反，通过离心收获的肉汤的浊度约为50-90 NTU，并且来自声学滤光器（Biosep）的收获流的浊度为33 NTU。这些结果表明，当使用VHU单元作为病毒收集过程模式时，可以消除二次澄清步骤。

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