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首页> 外文会议>IEEE Portuguese Meeting in Bioengineering >Towards a portable magnetoresistive biochip for urease-based biocementation monitoring*
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Towards a portable magnetoresistive biochip for urease-based biocementation monitoring*

机译：面向基于脲酶的生物胶结监测的便携式磁阻生物芯片 *

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In recent years there has been an exploitation of sustainable and environmental-friendly technologies for civil engineering applications. One of the most studied and promising technologies is soil improvement using biocementation through microbially induced calcite precipitation (MICP). MICP occurs due to the enzymatic activity of microbes living or added to the soil, with the analysis of this enzyme allowing a possible method for quantifying the amount of biocement (calcite) produced. This work presents and validates a novel detection tool to quantify and monitor urease in situ by employing a lab-on-a-chip (LOC) device. The device uses magnetoresistive biochips as biosensors in tandem with a read-out electronic set-up, magnetic labels and an integrated microfluidics system. A calibration curve for jack bean urease concentrations of 0.5 mg/mL to 70 mg/mL was obtained, serving as proof-of-concept for future in situ monitorization of biocementation. Experiments comparing direct urease assay detection versus sandwich assays were also conducted, with no difference being found between the two methodologies.
机译：近年来，已经在土木工程应用中开发了可持续的和环境友好的技术。研究最多且最有前途的技术之一是通过微生物诱导的方解石沉淀（MICP）的生物胶结法改良土壤。 MICP的产生是由于生活在土壤中或添加到土壤中的微生物的酶促活性，对这种酶的分析提供了一种可能的方法来量化所产生的生物水泥（方解石）的量。这项工作提出并验证了一种新颖的检测工具，可通过采用芯片实验室（LOC）装置现场量化和监测尿素酶。该设备将磁阻生物芯片用作生物传感器，并带有读出的电子装置，磁性标签和集成的微流控系统。获得了介于0.5 mg / mL至70 mg / mL的波罗的豆尿素酶浓度的校准曲线，可作为未来生物固结监测的概念验证。还进行了将直接脲酶测定与夹心测定进行比较的实验，两种方法之间没有发现差异。

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