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首页> 外文会议>ASME/JSME/KSME Joint Fluids Engineering Conference >HYDRODYNAMIC EVALUATION OF MICROTITER PLATE ASSAY USING COMPUTATIONAL FLUID DYNAMICS FOR BIOFILM FORMATION
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HYDRODYNAMIC EVALUATION OF MICROTITER PLATE ASSAY USING COMPUTATIONAL FLUID DYNAMICS FOR BIOFILM FORMATION

机译：利用计算流体动力学对微膜形成进行微量滴定板分析的水力评价

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Biofilms are complex surface associated communities where bacterial cells are enclosed by self-produced extra cellular polymeric substances (EPS), mainly consisting of exopolysaccharides, proteins and extracellular DNA. Treatment of biofilm associated persistent infections is an emerging issue for clinicians as bacterial cells adhere with human epithelial cells or indwelling medical devices such as implants and catheters, used in urinary tract and respiratory infections. Several methods are in practice to assess the biofilm formation of bacterial strains. Most of these are phenotypic methods which include Congo red assay (CRA), Air liquid interface (ALI), tissue culture plate method and Microtiter plate assay (MTPA). MTPA is considered as a standard screening method for comparing adherence pattern and is the most widely used quantitative method for detection of biofilm formation. Generally, the assay is performed under standard static conditions and little is known about the hydrodynamics in the microtiter plates. A few studies have applied computational fluid dynamics (CFD) simulations to describe flow pattern in microtiter plates during biofilm production and optimized the suitable conditions to detect the biofilm formation which have proven to be efficient. In this work the dependencies of biofilm formation on the hydrodynamics in microtiter plate assays were evaluated using OpenFOAM® an open-source toolbox for numerical simulation. It was found that higher flow rates increase the nutrient availability, promote cell growth, and attachment pattern with increased production of exopolymer, while the increase in flow velocity increases the shear rate causing erosion and disassembly of biofilm production because of detachment from the surface.
机译：生物膜是复杂的表面相关群落，细菌细胞被自身产生的细胞外聚合物质（EPS）包围，主要由胞外多糖，蛋白质和细胞外DNA组成。生物膜相关的持续性感染的治疗对于临床医生来说是一个新兴问题，因为细菌细胞会粘附在人上皮细胞或用于尿路和呼吸道感染的医疗设备（例如植入物和导管）中。在实践中，有几种方法可以评估细菌菌株的生物膜形成。这些大多数是表型方法，包括刚果红测定法（CRA），气液界面（ALI），组织培养板法和微量滴定板法（MTPA）。 MTPA被认为是比较粘附模式的标准筛选方法，是检测生物膜形成的最广泛使用的定量方法。通常，该测定是在标准静态条件下进行的，对微量滴定板中的流体动力学知之甚少。一些研究已应用计算流体动力学（CFD）模拟来描述生物膜生产过程中微量滴定板中的流动模式，并优化了已证明有效的检测生物膜形成的合适条件。在这项工作中，使用OpenFOAM®（用于数值模拟的开源工具箱）评估了微孔板测定中生物膜形成对流体动力学的依赖性。已经发现，较高的流速增加了营养素的利用率，促进了细胞的生长，并伴随着外聚物产量的增加而增加了附着模式，而流速的增加则增加了剪切速率，由于与表面的分离，导致了生物膜生产的腐蚀和分解。

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