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首页> 外文会议>International Bhurban Conference on Applied Sciences and Technology >First report on clinico-bacteriological examination of Canine Mastitis in Punjab
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First report on clinico-bacteriological examination of Canine Mastitis in Punjab

机译：旁遮普邦犬乳腺炎临床细菌学检查首次报告

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The present study primarily focuses on one of the most significant disease of the lactating bitches, known as Mastitis. Pet hospitals of four districts of Punjab were selected for the study. Total 400 bitches were examined and selected, 100 from each district for the positive percentage of Clinical and sub-clinical mastitis in canines. The presence of palpable changes in mammary gland of bitches and changes in milk secretion clearly diagnose the clinical mastitis The milk samples were collected from the lactating bitches were preserved in ice-box and sent to laboratory for the screening of clinical and sub-clinical mastitis via Surf Field Mastitis Test (SFMT). All the positive milk samples for canine mastitis were subjected to bacteriological examination via different bacterial culturing and staining techniques along with biochemical tests for the characterization and isolation bacteria. The blood samples were collected for the hematological examination and serum chemistry. The results shows that the overall positive prevalence of canine mastitis in Punjab was 56.25% where as in district Lahore was 46%, 49% in district Rawalpindi, 62% in district Faisalabad and 69% in district Multan. The overall percentage of clinical and sub-clinical mastitis in Punjab was 14.75% and 41.5% respectively. The bacteriological examination revealed the positive percentage of bacteria isolated from the milk were $E$. coli (25%), Staphylococcus aureus (39%), Streptococcus agalactia (11%) and Klebsiella spp (25%). The in-vitro susceptibility tests showed that the most effective susceptible drug against E. coli and Staph. aureus were Gentamycin and Ciprofloxacin whereas Augmentin showed the intermediate results. Similarly, for Strep. agalactiae and Klebsiella spp. the most susceptible antibiotic was Augmentin followed by the Gentamycin and Ciprofloxacin. Lincomycin was completely resistant to all bacterial isolates. The hematological values like RBCs, hemoglobin PCV and neutrophils decreased whereas the WBC level decreases to minimum normal range The electrolyte values of Ca, P, Cl, K showed the marked increase. No significant change was observed in the level of ALT, ALP and AST. The level of Albumin and Glucose showed slight decrease. The present study was helpful in the bacteriological characterization of canine mastitis along with understanding of antibiotic resistance in canines. It is also useful in determining the hematological values, serum chemistry and electrolyte balance of bitches during mastitis.
机译：本研究主要集中在哺乳母犬最重要的疾病之一，称为乳腺炎。研究选择了旁遮普邦四个地区的宠物医院。检查并选择了总共400只母犬，每个地区100只母犬中临床和亚临床乳腺炎的阳性百分比。母乳腺的明显变化和乳汁分泌的变化清楚地诊断了临床乳腺炎。从泌乳母乳中收集的牛奶样品保存在冰箱中，并通过实验室送往实验室进行临床和亚临床乳腺炎的筛查。冲浪场乳腺炎测试（SFMT）。通过不同的细菌培养和染色技术，对所有犬乳腺炎的阳性乳样品进行细菌学检查，并进行生化测试，以鉴定和分离细菌。收集血样用于血液学检查和血清化学检查。结果显示，旁遮普邦的犬乳腺炎总体阳性率为56.25％，而拉合尔地区为46％，拉瓦尔品第地区为49％，费萨拉巴德地区为62％，木尔坦地区为69％。旁遮普邦临床和亚临床乳腺炎的总百分比分别为14.75％和41.5％。细菌学检查显示，从牛奶中分离出的细菌的阳性百分比为 $ E $ 。大肠杆菌（25％），金黄色葡萄球菌（39％），无乳链球菌（11％）和克雷伯菌属（25％）。体外药敏试验表明，最有效的抗大肠埃希氏菌和葡萄球菌的药物。金黄色葡萄球菌为庆大霉素和环丙沙星，而奥古曼汀为中等结果。同样，对于链球菌。无乳杆菌和克雷伯菌属。最易感的抗生素是Augmentin，其次是庆大霉素和环丙沙星。林可霉素完全抵抗所有细菌分离株。红细胞，血红蛋白PCV和中性粒细胞等血液学值下降，而白细胞水平下降到最小正常范围。Ca，P，Cl，K的电解质值显着增加。 ALT，ALP和AST的水平未见明显变化。白蛋白和葡萄糖水平略有下降。本研究有助于犬乳腺炎的细菌学表征以及对犬类抗生素耐药性的了解。在确定乳腺炎期间母犬的血液学价值，血清化学和电解质平衡方面也很有用。

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