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首页> 外文会议>Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V >Protoporphyrin IX distribution after intra-articular and systemic application of 5-aminolevulinic acid in healthy and arthritic joints
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Protoporphyrin IX distribution after intra-articular and systemic application of 5-aminolevulinic acid in healthy and arthritic joints

机译：在健康和关节炎关节内和全身应用5-氨基乙酰丙酸后原卟啉IX分布

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Abstract: Arthroscopic synovectomy, which is limited today to thelarge joints, is an important early treatment ofrheumatoid arthritis (RA). Photodynamic therapy (PDT)is potentially to be a less invasive method of removingthe synovial membrane. Therefore, in a rabbit model ofRA, the accumulation of the photosensitizerProtoporphyrin IX (PPIX) after intra-articular andsystemic application of ALA into arthritic rabbit kneejoints was studied in skin, patella, synovial tissue,and meniscus by fluorescence microscopy. PPIXfluorescence was measured in biopsies taken atdifferent times after application of neutral and acidALA solutions. Significant PPIX fluorescence wasobserved in the synovial membrane and skin 2 and 4hours after application. Using intra-articularapplication, ALA solutions prepared with pH 5.5 were atleast as efficient as neutral solutions in sensitizingthe synovial membrane. Skin also showed PPIX within 4hours after application. After 24 hours, a marginalPPIX fluorescence was detected in these tissues. On theother hand, in cartilage and meniscus significant PPIXaccumulation was still observed 24 hours after ALAinjection. Systemic application of ALA also showed agood accumulation of PPIX. Further experiments areneeded to show whether accumulation of thephotosensitizer and tissue selectivity are sufficientfor a successful treatment of rheumatoid synovitis. !8
机译：【摘要】关节镜滑膜切除术目前仅限于大关节，是风湿性关节炎（RA）的重要早期治疗方法。光动力疗法（PDT）可能是一种去除滑膜的侵入性较小的方法。因此，在兔RA模型中，通过荧光显微镜研究了在关节炎，兔膝关节的关节内和全身应用ALA后在皮肤，骨，滑膜组织和半月板中光敏剂原卟啉IX（PPIX）的蓄积。应用中性和酸性ALA溶液后，在不同时间进行的活检中测量PPIX荧光。施药后2和4小时，在滑膜和皮肤中观察到了显着的PPIX荧光。使用关节内应用，用pH 5.5制备的ALA溶液在使滑膜增敏方面至少与中性溶液一样有效。涂抹后4小时内皮肤也显示出PPIX。 24小时后，在这些组织中检测到边缘PPIX荧光。另一方面，在ALA注射后24小时，在软骨和半月板中仍观察到明显的PPIX蓄积。全身应用ALA也显示出良好的PPIX积累。需要进一步的实验以显示光敏剂的积累和组织选择性是否足以成功治疗类风湿性滑膜炎。 ！8

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