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首页> 外文会议>Conference on lasers in surgery: Advanced characterization, therapeutics, and systems XI >Granulation tissue exhibits differences in alpha-smooth muscle actin expression after laser assisted skin closure (LASC).
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Granulation tissue exhibits differences in alpha-smooth muscle actin expression after laser assisted skin closure (LASC).

机译：激光辅助皮肤闭合（LASC）后，肉芽组织在α平滑肌肌动蛋白表达中表现出差异。

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Introduction: Laser Assisted Skin Closure (LASC) has been shown to accelerate wound healing and to reduce scarring. However, the mechanisms involved during the cutaneous wound repair after LASC remain unclear. This study aimed to evaluate the inflammation process and the wound repair fibroplasia, particularly the involvement of granulation tissue fibroblasts (myofibroblasts). Study Design/Materials and Methods: four dorsal skin incisions were performed on back of male hairless rats (n=3). For each wound, the good apposition of the edges was obtained with buried absorbable suture. Incisions were closed by 3 different techniques: 1-Transparent Adhesive Dressing (TAD) alone, 2-Continuous Intradermal Suture (CIDS) + TAD, 3-TAD + LASC using 145J/cm~2. Similarly, the laser was applied without incision (4-Laser Alone). The last group (no laser - no incision) was used as control (5-CTRL). Clinical examination, standard histological study (Haematoxylin-Eosin-Safran) and immunohistochemical study (monoclonal antibody directed against Alpha Smooth Muscle Actin) were performed at 3 and 7 days. The densities of inflammation and immunoreactivity were qualitatively appreciated through an arbitrary scale from 0 to 3 (0=undetected, 1=low density, 2=medium density, 3=dense) in dermis and hypodermis. Results: In the laser group, healing was acclerated when compared to the CIDS and TAD groups. When compared to the CIDS and TAD groups, LASC samples display less inflammation in hypodermis. An increased number of myofibroblasts is seen in dermis and hypodermis at Day 3 and Day 7. Conclusion: This study clearly demonstrates that cutaneous wound repair process is modified after LASC leading to a scarless repair This study clearly demonstrates that cutaneous wound repair process is modified after LASC leading to a scarless repair This observation is in accordance with our previous clinical findings and could suggest a novel therapeutic approach to reduce scar formation after surgery.
机译：简介：激光辅助皮肤闭合术（LASC）可以加速伤口愈合并减少疤痕。然而，在LASC术后皮肤伤口修复过程中涉及的机制仍不清楚。这项研究旨在评估炎症过程和伤口修复性纤维化，特别是肉芽组织成纤维细胞（肌成纤维细胞）的参与。研究设计/材料和方法：在雄性无毛大鼠（n = 3）的背部进行四个背侧皮肤切口。对于每个伤口，利用可吸收的埋入式缝合线可得到边缘的良好贴合。通过3种不同的技术闭合切口：1单独使用透明胶粘剂敷料（TAD），2连续皮内缝合（CIDS）+ TAD，3 TAD + LASC（使用145J / cm〜2）。类似地，没有切口地施加激光（单独使用4激光）。最后一组（无激光-无切口）用作对照（5-CTRL）。在第3天和第7天进行了临床检查，标准组织学研究（苏木精-伊红-番红花）和免疫组织化学研究（针对Alpha平滑肌肌动蛋白的单克隆抗体）。通过在真皮和皮下组织中从0到3（0 =未检测到，1 =低密度，2 =中密度，3 =密集）的任意比例定性地认识到炎症和免疫反应的密度。结果：与CIDS和TAD组相比，激光组的愈合得以加快。与CIDS和TAD组相比，LASC样品在皮下的炎症较少。在第3天和第7天，在真皮和皮下组织中发现的成肌纤维细胞数量增加。结论：这项研究清楚地表明，LASC后皮肤伤口修复过程被修饰，从而实现了无疤的修复。这项研究清楚地表明，皮肤创面修复过程在经过LASC后得到了修饰。导致无疤痕修复的LASC该观察结果与我们先前的临床发现一致，并且可能提出了一种减少手术后疤痕形成的新颖治疗方法。

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