首页> 外文会议>Instrumentation and Measurement Technology Conference, 2002. IMTC/2002. Proceedings of the 19th IEEE >Instrumental method for quantitative evaluation of cell/particle adhesion based on transport measurements in capillary flow. Part I: The measuring system
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Instrumental method for quantitative evaluation of cell/particle adhesion based on transport measurements in capillary flow. Part I: The measuring system

机译:基于毛细管流中转运测量值定量评估细胞/颗粒粘附的仪器方法。第一部分:测量系统

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An instrumental method is proposed for quantitative evaluation of cell/particle adhesion at solid/liquid interfaces. The retardation of convective cell/particle transport is measured in capillary flow tubes (columns), that have been internally coated with the substance under investigation. Ac-operated electronic gating is used for the determination of the elution rate. The technique is similar to that of HPLC (high pressure liquid chromatography). Cell/particle transport however, on column, is totally different from that of solutes: transport mainly takes place at a radial distance about halfway the axis and the wall (Segre-Silberberg effect). At specific flow rates of the carrier, cells/particles contact the wall and may be released again as a consequence of increasing lift and drag. The resulting transport retardation depends on the chemical nature of the capillary wall coatings.
机译:提出了一种用于定量评估固/液界面处细胞/颗粒粘附性的仪器方法。对流细胞/颗粒传输的阻滞是在毛细管流动管(柱)中测量的,该毛细管内部已被研究物质覆盖。交流电子门控用于确定洗脱速率。该技术类似于HPLC(高压液相色谱)。但是,在柱上的细胞/颗粒运输与溶质完全不同:运输主要发生在大约距离轴和壁一半的径向距离处(Segre-Silberberg效应)。在特定的载体流速下,细胞/颗粒会与壁接触,并可能由于升力和阻力的增加而再次释放。产生的传输延迟取决于毛细管壁涂层的化学性质。

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