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首页> 外文会议>Water quality technology conference >Detecting Enteroviruses Using Integrated Cell Culture-PCR and Serotyping Male-specific Coliphages Isolates in Water
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Detecting Enteroviruses Using Integrated Cell Culture-PCR and Serotyping Male-specific Coliphages Isolates in Water

机译：使用集成的细胞培养-PCR和血清分型男性特定鹅毛病分离株检测肠病毒

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A quantitative method to detect infectious enteroviruses in water by integrated cell culture-PCR(ICC-PCR) was evaluated. Of 9 water samples, 7 were positive for enteroviruses using ICC-PCR.The concentrations of enteroviruses were calculated by the Most Probable Number (MPN) methodand ranged from 33 to 189 MPN/100 L. ICC-PCR-MPN is a very sensitive and rapid method toquantify infectious enteroviruses in water. Male-specific coliphages (F~+) were detected in watersamples using single agar layer (SAL) and direct membrane filtration (DMF). Double agar layer(DAL) method was used to detect F~+ coliphages in concentrated water samples. All samples werepositive for male-specific coliphages, ranged from 3 to 180 pfu/100 mL. Correlation (r = 0.61)existed between the presence of enteroviruses and male-specific coliphages in concentrated watersamples. A total of 61 male-specific coliphage isolates were serotyped to determine theirserogroups. The results indicated that 74% were RNA phages with the majority being groups I andII. However, group III male-specific coliphages were detected in all sites, indicating humansources were likely to be contributing some of the fecal contamination.
机译：集成细胞培养-PCR定量检测水中感染性肠病毒的方法 （ICC-PCR）被评估。在9个水样中，有7个使用ICC-PCR检测到肠病毒呈阳性。 肠病毒的浓度通过最可能数（MPN）方法计算 范围从33到189 MPN / 100L。ICC-PCR-MPN是一种非常灵敏，快速的检测方法 定量水中的传染性肠病毒。在水中检测到雄性特异性噬菌体（F〜+） 样品使用单琼脂层（SAL）和直接膜过滤（DMF）。双琼脂层 （DAL）方法用于检测浓缩水样品中的F〜+噬菌体。所有样品均为 男性特异性大肠杆菌噬菌体呈阳性，范围从3至180 pfu / 100 mL。相关性（r = 0.61） 在浓缩水中存在肠病毒和雄性特异噬菌体之间存在 样品。总共对61种男性特异性大肠杆菌噬菌体进行了血清分型，以确定它们 血清群。结果表明74％是RNA噬菌体，大多数是I组和 二。但是，在所有位点都检测到了第三组雄性特异性噬菌体，表明人类 污染源可能是造成粪便污染的原因之一。

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