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首页> 外文会议>国际营养科学联盟第八届临床营养学大会暨第五届亚太临床营养学会大会 >Antioxidant and protective effect of an oleanolic acid-enriched extract of A. deliciosa root on carbon tetrachloride induced rat liver injury
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Antioxidant and protective effect of an oleanolic acid-enriched extract of A. deliciosa root on carbon tetrachloride induced rat liver injury

机译：富含齐墩果酸的提取物曲霉根提取物对四氯化碳诱导的大鼠肝损伤的抗氧化和保护作用

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The ethanol-water extract of A. deliciosa root (EEAD) was fractionated into n-hexane (EEAD-He), ethyl acetate (EEAD-Ea), n-butanol (EEAD-Bu) and aqueous (EEAD-Aq) fractions according to their different polarity and solubility. Among the four extracts, it was found that EEAD-Bu was enriched with oleanolic acid (OLA). The antioxidant and hepatoprotective activities of various EEAD fractions and OLA were carefully investigated by the methods of ferric thioeyanate (FTC) and thiobarbituric acid (TBA), as well as the model of CCL4-induced liver toxicity in rats. The results showed that the EEAD-Bu had higher in vitro antioxidant and in vivo hepatoprotective activities than those of the other types of extracts (p＜0.05). When the CCL4-induced rats were treatment with 120 mg/kg EEAD-Bu, the activities of alanine transaminase (ALT) and aspartate transanimase (AST) in rat serum decreased 90 % and 81%, respectively, as compared with those of the CCL4 control rats. Furthermore, the lipid peroxidation (MDA) decreased 42 % and glutathione (GSH) increased 114 % in the rats liver homogenate, as compared with those of the control. The results also indicated that the hepatoprotective activity of the EEAD-Bu (at the dose of 120 mg/kg) was higher than that of the reference drug silymarin (at the dose of 60 mg/kg), and OLA acted as an important role in dose-dependent protection against CCL4 hepatotoxicity. The findings indicate that the OLA-enriched EEAD-Bu extract had significant and concentration dependent hepatoprotective effect for the carbon tetrachloride induced rat liver injury.
机译：将A. deliciosa根（EEAD）的乙醇-水提取物分馏为正己烷（EEAD-He），乙酸乙酯（EEAD-Ea），正丁醇（EEAD-Bu）和水相（EEAD-Aq）馏分使其具有不同的极性和溶解性。在这四种提取物中，发现EEAD-Bu富含齐墩果酸（OLA）。通过硫氰酸铁（FTC）和硫代巴比妥酸（TBA）的方法，以及CCL4诱导的大鼠肝毒性模型，仔细研究了各种EEAD馏分和OLA的抗氧化和保肝活性。结果表明，EEAD-Bu具有比其他类型提取物更高的体外抗氧化剂和体内保肝活性（p ＜0.05）。当用CCL4诱导的大鼠用120 mg / kg EEAD-Bu处理时，与CCL4相比，大鼠血清中丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的活性分别降低了90％和81％。对照大鼠。此外，与对照组相比，大鼠肝脏匀浆的脂质过氧化（MDA）降低了42％，谷胱甘肽（GSH）升高了114％。结果还表明，EEAD-Bu（120 mg / kg剂量）的肝保护活性高于参考药物水飞蓟素（60 mg / kg剂量），并且OLA发挥了重要作用对CCL4肝毒性具有剂量依赖性保护作用。研究结果表明，富含OLA的EEAD-Bu提取物对四氯化碳诱导的大鼠肝损伤具有显着且浓度依赖性的肝保护作用。

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