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Comparison between optical measurements made from natural and frozen samples at optical clearing

机译:光学清洁下的自然和冷冻样品中的光学测量比较

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To determine the differences between the optical clearing effects created by ethylene glycol in fresh and frozen samples, we have performed several measurements from samples in both conditions. Fresh samples were used after animal sacrifice and frozen samples were kept at -20°C for 72 hours. The different measurements performed with samples from both cases were total transmittance, collimated transmittance, total reflectance and specular reflectance. Considering, for instance, collimated transmittance measurements, we have verified that the spectra measured from both samples before adding the solution present different levels of collimated transmittance. The time-dependence evolution of the collimated transmittance spectrum is similar between both cases of samples, but since they present different levels of "natural" transmittance, the optical clearing effect is observed at different levels if we compare between fresh and frozen samples.
机译:为了确定新鲜和冷冻样品中的乙二醇产生的光学清除效果之间的差异,我们已经从两种条件下进行了几次测量。在动物牺牲和冷冻样品在-20℃下保持72小时后使用新鲜样品。使用两种情况的样品进行的不同测量值是总透射率,准直透射率,总反射率和镜面反射率。例如,考虑到准直透射率测量,我们已经验证了在添加解决方案之前从两个样品测量的光谱呈现不同水平的准直透射率。准直透射谱的时间依赖性演化在两种样品之间类似,但由于它们呈现不同水平的“自然”透射率,如果我们在新鲜和冷冻的样品之间比较,则在不同的级别观察到光学清除效果。

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