• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Meeting on Cell Signaling World: Signal Transduction Pathways as Therapeutic Targets >Breast Cancer Cells Response to the Antineoplastic Agents Cisplatin, Carboplatin, and Doxorubicin at the mRNA Expression Levels of Distinct Apoptosis-Related Genes, Including the New Member, BCL2L12
【24h】

Breast Cancer Cells Response to the Antineoplastic Agents Cisplatin, Carboplatin, and Doxorubicin at the mRNA Expression Levels of Distinct Apoptosis-Related Genes, Including the New Member, BCL2L12

机译：在不同凋亡相关基因（包括新成员BCL2L12）的mRNA表达水平上，乳腺癌细胞对抗肿瘤药顺铂，卡铂和阿霉素的反应

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Most apoptosis-related genes regulate cellular fate as a re sponse to anticancer drugs. Modulations at the mRNA levels of such genes often correlate with the sensitivity of various types of cancer cells to chemotherapeutic reagents. The drugs cisplatin, carboplatin, and dox orubicin exhibit anticancer activity, the mechanism of which is not yet completely clarified, although they are known to modulate the expression of several genes including apoptosis-related genes, such as members of the BCL2 (Bcl-2) family. In order to define the significance of the expression patterns of such genes as a response to anticancer drug cytotoxic activity, we studied the possible alterations in the mRNA expression levels of var ious apoptosis-related genes, including the new member, BCL2L12, after cell treatment with distinct anticancer drugs (cisplatin, carboplatin, and doxorubicin), in the breast cancer cell line, MCF-7. The kinetics of cell toxicity was evaluated by the MTT method, whereas the expression levels of distinct apoptosis-related genes were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR), using gene-specific primers. The percentage of nonviable cells was upregulated with increasing concentra tions and cell exposure time to the different anticancer drugs. Distinct modulations of apoptosis-related genes, at the mRNA level, were also ob served. However, further work is required in order to ascertain whether the mRNA expression profile of such genes may provide evidence for their contribution to more specific and sensitive prediction of breast cancer re sponse to treatment and therefore the rationale for individualized, more appropriate, and successful treatment.
机译：大多数与凋亡相关的基因作为抗癌药物的反应调节细胞命运。这种基因的mRNA水平上的调节常常与各种类型的癌细胞对化学治疗剂的敏感性相关。尽管已知顺铂，卡铂和多柔比星具有抗癌活性，但其机制尚未完全阐明，尽管它们可以调节包括凋亡相关基因在内的几种基因的表达，例如BCL2（Bcl-2）的成员。 ） 家庭。为了确定此类基因表达模式作为对抗癌药物细胞毒活性的反应的重要性，我们研究了细胞处理后各种凋亡相关基因（包括新成员BCL2L12）的mRNA表达水平可能发生的变化在乳腺癌细胞系MCF-7中具有独特的抗癌药物（顺铂，卡铂和阿霉素）。通过MTT方法评估细胞毒性的动力学，而使用基因特异性引物通过逆转录酶聚合酶链反应（RT-PCR）分析不同凋亡相关基因的表达水平。随着浓度增加和不同抗癌药物的细胞暴露时间，无活力细胞的百分比上调。还观察到了与凋亡相关的基因在mRNA水平上的不同调节。但是，需要做进一步的工作以确定这种基因的mRNA表达谱是否可以为它们对乳腺癌对治疗的反应做出更具体，更敏感的预测提供证据，从而确定个体化，更合适和成功治疗的理由。 。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号