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Digital pinhole filter used for detection of confocal microscopy images

机译:用于检测共聚焦显微镜图像的数字针孔滤光片

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When conventional physical pinholes are used, the alignment of pinholes is always a problem. An iterative Wiener deconvolution filter is used to reject out-of-focus light, a sub-pixel centre location method is used to locate the centre of the pinhole, and the dimensions of the pinhole are optimized using the confocal microscopic theory. A filter with digital pinhole is thus developed and compared with filter with conventional physical pinhole through comparative tests. Experimental results indicate that the alignment of pinholes can be made easy, a filter with digital pinhole can be used to take place of a filter with conventional physical pinhole, and it can also reduce the pixel crosstalk resulting from multipoint illumination.
机译:当使用常规的物理针孔时,针孔的对准始终是一个问题。使用迭代的维纳反卷积滤波器来拒绝散焦光,使用子像素中心定位方法来定位针孔的中心,并使用共聚焦显微镜理论对针孔的尺寸进行优化。因此,开发了具有数字针孔的滤波器,并通过对比测试将其与具有常规物理针孔的滤波器进行了比较。实验结果表明,可以简化针孔的排列,可以使用具有数字针孔的滤光片代替具有常规物理针孔的滤光片,并且还可以减少多点照明引起的像素串扰。

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