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首页> 外文会议>2009 ISEST;International symposium on environmental science and technology >Isolation of Atrazine-degrading Arthrobacter sp. AD30 and Pseudomonas sp. AD39 and Their Application in Biotreatment of Industrial Wastewater
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Isolation of Atrazine-degrading Arthrobacter sp. AD30 and Pseudomonas sp. AD39 and Their Application in Biotreatment of Industrial Wastewater

机译：降解阿特拉津的节杆菌的分离AD30和假单胞菌AD39及其在工业废水生物处理中的应用

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By enrichment culture two bacterial strains, AD30 and AD39, capable of utilizing atrazine as a sole nitrogen source for growth were isolated from a mixture of industrial wastewater and sludge from an atrazine manufacturing plant. 16S rRNA gene sequencing identified AD30 as Arthrobacter sp. and AD39 as Pseudomonas sp. The composition of atrazine-degrading genes and degradation characteristic of Arthrobacter sp. AD30 and Pseudomonas sp. AD39 were studied in detail. PCR assays indicated that both AD30 and AD39 contained atrazine-degrading genes trzN and atzBC and degraded toxic atrazine to nontoxic cyanuric acid. The biodegradation experiments indicated that after minimal media containing 200 mg/L atrazine were inoculated separately with AD30, AD39 and their mixture and incubated at 30 with shaking for 48 hours atrazine were removed by 92.5 °C ％, 97.9％ and 99.6％, respectively. This showed that atrazine degradation of the mixed bacteria was better than single strain. After industrial wastewater containing 176 mg/L atrazine was inoculated with the mixed bacteria and incubated at 30 with shaking for 72 hours atrazine were removed by 99.1 °C ％, nominating the mixed bacteria as a good candidate for use in biotreatment of atrazine industrial wastewater.
机译：通过富集培养，两种细菌菌株，AD30和AD39能够利用阿特拉津作为唯一的氮源进行生长的唯一氮源，从亚特津制造厂的污泥的混合物中分离出生长的生长。 16S RRNA基因测序鉴定AD30作为Arthrobacter SP。和ad39作为pseudomonas sp。亚特拉嗪降解基因的组成和梭菌SP的降解特征。 AD30和Pseudomonas sp。详细研究了AD39。 PCR测定表明，AD30和AD39均含有阿特拉嗪降解基因TrzN和ATZBC，并降解了无毒的氰尿酸。生物降解实验表明，在用Ad30，Ad39及其混合物中分别接种含有200mg / L atrazine的最小培养基，并在30小时内孵育48小时，分别通过92.5°C％，97.9％和99.6％除去亚特津。这表明混合细菌的阿特拉啶降解优于单株。含有混合细菌的含有176mg / L阿特拉嗪的工业废水并用振荡温育72小时，通过99.1°C％除去阿特拉嗪，提名混合细菌作为阿特拉津产业废水生物处理的良好候选者。

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