THERMAL PRECONDITIONING PROTOCOLS FOR CARTILAGE TISSUE ENGINEERING

机译：软骨组织工程的热预处理协议

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This study focused on identifying effective thermal stress preconditioning protocols for inducing HSP70 expression for cartilage tissue engineering. Cell viability and HSP70 expression levels were quantified for several heating protocols. Cell viability remained high without alterations in cellular morphology for heating durations shorter than 8 min at 44°C. HSP70 expression increased with higher temperatures and for longer heating durations. However, additional stress preconditioning protocols should be explored and correlated with expression of other relevant HSPs, cell viability, cell proliferation, and formation of extracellular matrix such as collagen and aggrecan to develop optimization of heat stress conditioning.

机译：这项研究的重点是确定有效的热应激预处理方案，以诱导软骨组织工程中HSP70的表达。对于几种加热方案，对细胞活力和HSP70表达水平进行了定量。在44°C下加热时间短于8分钟的情况下，细胞活力仍然很高，而细胞形态没有改变。 HSP70表达随着温度的升高和加热时间的延长而增加。但是，应探索其他应激预处理方案，并将其与其他相关HSP的表达，细胞活力，细胞增殖以及细胞外基质（例如胶原蛋白和聚集蛋白聚糖）的形成相关联，以开发热应激条件优化。

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《ASME summer bioengineering conference;SBC2008》|2009年|655-656|共2页
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Eunna Chung; M. N. Rylander;
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tissue engineering; cell viability; chondrocyte; heat shock protein; heat stress;

机译：组织工程;细胞活力;软骨细胞热激蛋白热应激;

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1. Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering [J] . Schneider Cornelia, Lehmann Johannes, van Osch Gerjo J. V. M., Tissue engineering, Part C. Methods . 2016,第12期

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2. Mechanical Stimulation Protocols of Human Derived Cells in Articular Cartilage Tissue Engineering - A Systematic Review [J] . Khozoee Baktash, Mafi Pouya, Mafi Reza, Current stem cell research & therapy . 2017,第3期

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3. Investigation of microstructure, mechanical properties and cellular viability of poly(L-lactic acid) tissue engineering scaffolds prepared by different thermally induced phase separation protocols. [J] . Sara Molladavoodi, Maud Gorbet, John Medley, Journal of the mechanical behavior of biomedical materials . 2013,第Null期

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4. THERMAL PRECONDITIONING PROTOCOLS FOR CARTILAGE TISSUE ENGINEERING [C] . ASME bioengineering conference . 2009

机译：软骨组织工程热预处理方案
5. Preconditioning methods in cartilage tissue engineering: Influences of silk material properties and hypoxia on chondrogenesis. [D] . Yodmuang, Supansa. 2013

机译：软骨组织工程中的预处理方法：丝材料特性和缺氧对软骨形成的影响。
6. A Hyaluronic Acid Based Injectable Hydrogel Formed via Photo-Crosslinking Reaction and Thermal-Induced Diels-Alder Reaction for Cartilage Tissue Engineering [O] . Gang Wang, Xiaodong Cao, Hua Dong, 2018

机译：通过光交联反应和热诱导的Diels-Alder反应形成的透明质酸可注射水凝胶用于软骨组织工程。
7. Preconditioning Methods in Cartilage Tissue Engineering: Influences of Silk Material Properties and Hypoxia on Chondrogenesis [O] . Yodmuang Supansa 2013

机译：软骨组织工程中的预处理方法：丝材料特性和缺氧对软骨形成的影响

THERMAL PRECONDITIONING PROTOCOLS FOR CARTILAGE TISSUE ENGINEERING

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