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首页> 外文会议>IMECE2008;ASME international mechanical engineering congress and exposition >MEASUREMENT OF DYNAMIC VISCOELASTICITY OF AN OSTEOBLAST UNDER ADHESIVE CONDITION USING A PIEZOELECTRIC VIBRATOR
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MEASUREMENT OF DYNAMIC VISCOELASTICITY OF AN OSTEOBLAST UNDER ADHESIVE CONDITION USING A PIEZOELECTRIC VIBRATOR

机译：压电振动器在黏性条件下测量成骨细胞的动态粘弹性

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Bone cells are adaptive to surrounding mechanical conditions. Osteoblasts, one of bone cells, have been reported to be sensible to mechanical stimulation and change the generated bone mass. Viscoelastic properties of such cells are predicted to be related to this phenomenon in the view of mechanical dynamics. In order to find the effective stimulation on the bone formation, it is necessary to understand the viscoelastic properties of the cells. Especially in the case of bone cells, it is important to consider their adhesive condition because they attach on surfaces of bone matrices. In this study, we measured dynamic viscoelastic properties of a cultured osteoblast, MC3T3-E1, under adhesive condition. Using the experimental results, we derived a model for viscoelasticity of the cell and identified the value of each element in this model.The cells were seeded on a glass plate in a petri dish. After the cells were cultured for one day and adhered on the glass plate, it was vertically raised and fixed on a piezo actuator. The center of the cell surface was aspirated with an L-shaped micropipette to be held. The glass plate was moved with the piezo actuator. The load applied to the cell was obtained by measuring the deflection of the micropipette whose spring constant was calibrated after each test. Deflection of the micropipette and elongation of the cell were measured by captured image during the test.As a result, the dynamic viscoelasticity of the cells was measured and modeled, and the value of each element in this model was identified.
机译：骨细胞适应周围的机械状况。据报道，成骨细胞是骨细胞之一，对机械刺激和改变产生的骨质很敏感。从机械动力学的角度来看，预计这种细胞的粘弹性能与这种现象有关。为了找到对骨形成的有效刺激，有必要了解细胞的粘弹性质。特别是在骨细胞的情况下，重要的是要考虑它们的粘附状况，因为它们附着在骨基质的表面上。在这项研究中，我们测量了粘附条件下培养的成骨细胞MC3T3-E1的动态粘弹性。使用实验结果，我们导出了细胞的粘弹性模型，并确定了该模型中每个元素的值。 将细胞接种在培养皿中的玻璃板上。将细胞培养一天并粘附在玻璃板上后，将其垂直升高并固定在压电致动器上。用L形微量移液管吸出细胞表面的中心。用压电致动器移动玻璃板。通过测量微量移液器的挠度来获得施加到细胞上的负载，每个微量移液器的弹性常数在每次测试后均已校准。在测试过程中，通过捕获的图像来测量微量移液器的变形和细胞的伸长。 结果，对细胞的动态粘弹性进行了测量和建模，并确定了该模型中每个元素的值。

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