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首页> 外文会议>2010 International Conference on Mechanic Automation and Control Engineering >A leaky pear S-allele was revealed by a new primer set and characterized by bioinformatics analysis
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A leaky pear S-allele was revealed by a new primer set and characterized by bioinformatics analysis

机译：一种新的引物组揭示了一个渗漏的梨S等位基因，并通过生物信息学分析对其进行了表征

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摘要

Pear is a commercially important fruit tree widely distributed in China that displays GSI (gametophytic self-incompatibility). It is necessary to determine S-alleles and S-genotypes for clarifying the compatibility between cultivars prior to pear planting and breeding. The S-genotype analysis using conventional method of artificial pollination is time consuming and requires much labor. In addition, the results obtained are often ambiguous due to environmental factors. PCR-based molecular method, however, could rapidly and accurately identified S-alleles and S-genotypes of pear cultivars. In this study, a new SXH-Rnase was cloned in Xuehua pear that was previously S-genotyped S4S16. Interestingly, the pear S-RNase consensus primers PF1 and PR1 failed to amplify product from the genomic DNA of Xuehua pear although they well matched the cDNA sequence of SXH-Rnase. To detect SXH-RNase from genomic DNA, primer pair of SXHSPF and SXHSPR were designed and successfully amplified one SXH-fragment of 408 bp containing a complete intron of 330 bp. In addition, the new SXH-Rnase was characterized in detail by bioinformatics analysis. This study is useful for pear planting, breeding and revealing the mechanism of GSI.
机译：梨是一种在商业上很重要的果树，在中国广泛分布，表现出GSI（配子体自我不相容性）。在梨种植和育种之前，有必要确定S等位基因和S基因型以阐明品种之间的相容性。使用常规的人工授粉方法进行S基因型分析是费时的并且需要大量的劳动。另外，由于环境因素，所获得的结果常常是模棱两可的。然而，基于PCR的分子方法可以快速，准确地鉴定梨品种的S等位基因和S基因型。在这项研究中，在雪梨中克隆了一个新的S XH -Rnase，该梨以前是S基因型的S4S16。有趣的是，尽管梨S-RNase共有引物PF1和PR1与S XH -Rnase的cDNA序列完全匹配，但它们未能从雪梨的基因组DNA扩增产物。为了从基因组DNA中检测S XH -RNase，设计了SXHSPF和SXHSPR引物对，并成功扩增了一个408 bp的S XH 片段，其中包含330 bp的完整内含子。此外，通过生物信息学分析对新的S XH -Rnase进行了详细表征。这项研究对于梨的种植，育种和揭示GSI的机理是有用的。

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