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Isolation and Identification of Imazethapyr-Degrading Actinomyces and Its Degradation Characteristics

机译:降解咪唑乙烟的放线菌的分离鉴定及其降解特性

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Imazethapyr is used widely all over the world for the control of broadleaf weeds in the soybean field. However the serious problem of Imazethapyr has been remainded for long time. Bioremediation is an effective and economic method for the soil that has been polluted by pesticides. Used method of bottle cultivating, one strain of Imazethapyr degradable actinomyces S180 was isolated from the soil where Imazethapyr has been applied for a long-term. The strain could utilize Imazethapyr as sole nitrogen for growth. This strain was related and shared characteristics to genus Streptomyces zaomyceticus, according to the physiological and biochemical properties as well as 16S rRNA sequence analysis. It found that 85.41% Imazethapyr was degraded by S180 in 6 days with 3% Imazethapyr in mineral medium under the optimal containing 200 mg · L-1 Imazethapyr, 30°C and pH 7. 0.
机译:咪唑乙烟碱在世界范围内广泛用于防治大豆田中的阔叶杂草。但是,伊马西ap的严重问题长期存在。对于被农药污染的土壤,生物修复是一种有效而经济的方法。使用瓶培养的方法,从长期施用Imazethapyr的土壤中分离出一种Imazethapyr可降解放线菌S180菌株。该菌株可以利用咪唑乙烟作为唯一的氮来生长。根据生理和生化特性以及16S rRNA序列分析,该菌株与zaomyceticticus属相关并具有共同特征。结果发现,在含有200 mg·L -1 咪唑乙烟醚,30°C和pH 7. 0的最适条件下,矿物培养基中的3%咪唑乙烟酚在6天内被S180降解了85.41%的咪唑乙烟醚。

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