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首页> 外文会议>2010 4th International Conference on Bioinformatics and Biomedical Engineering >Biotransformation of DL-2-Amino-Delta2;-Thiazoline-4-Carbonic Acid to L-Cysteine Using a Newly Isolated Pseudomonas sp. Zjwp-14
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Biotransformation of DL-2-Amino-Delta2;-Thiazoline-4-Carbonic Acid to L-Cysteine Using a Newly Isolated Pseudomonas sp. Zjwp-14

机译：使用新分离的假单胞菌属物种将DL-2-Amino-Delta2; -Thiazoline-4-Carbonic acid转化为L-半胱氨酸。 Zjwp-14

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A bacterial strain Pseudomonas sp. Zjwp-14 was screened out from soil, and used for the biotransformation of DL-2-amino-∆2-thiazoline-4-carbonic acid to L-cysteine. This strain was identified based on its morphological, physiological characterization, 16S rDNA sequence determination and phylogenetic analysis. The bioconversion process was optimized by examining some key factors including cell cultivation time, buffer pH, metal ions, aeration (anaerobic/aerobic) and substrate concentration. L-cysteine productivity reached 4.24 g•l-1 in 6 h under optimal conditions: cells were cultivated for 12 h, and then resuspended in pH 8.0 phosphate buffer contationing 10 g•l-1 of DL-ATC as substrate, with the addition of 2 mM MnSO4•4H2O, incubated in still culture flashing with N2 atmosphere at 42 oC for 6 h. The results suggested that Pseudomonas sp. Zjwp-14, a bacterial isolate, has applied potential for enzymatic production of L-cysteine.
机译：细菌菌株假单胞菌（Pseudomonas sp。）。从土壤中筛选出Zjwp-14，并将其用于DL-2-氨基-∆2-噻唑啉-4-碳酸向L-半胱氨酸的生物转化。该菌株是根据其形态，生理特征，16S rDNA序列测定和系统发育分析进行鉴定的。通过检查一些关键因素来优化生物转化过程，这些关键因素包括细胞培养时间，缓冲液pH值，金属离子，通气（厌氧/好氧）和底物浓度。在最佳条件下，L-半胱氨酸生产率在6小时内达到4.24 g•l-1：将细胞培养12 h，然后重悬于pH 8.0磷酸盐缓冲液中，以10 g•l-1的DL-ATC作为底物，并添加取2 mM MnSO4•4H2O，在静态培养中于42 oC的N2气氛中闪蒸6 h。结果表明假单胞菌属。 Zjwp-14，一种细菌分离物，具有酶促生产L-半胱氨酸的潜力。

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