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Assessment of genotoxicity of nitrobenzene using Vicia-micronucleus test

机译:用蚕豆微核试验评估硝基苯的遗传毒性

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The genotoxicity of nitrobenzene was evaluated by Vicia faba micronuclei (MN) assay, chromosomal aberration assay and mitotic index assay. Vicia faba primary root tip cell mitotic index (MI) increased following NB concentration from 5 mg/L to 50 mg/L. It implies that NB may effect on the mitosis of Vicia faba root tip cells, promote at lower concentration and inhibit at higher concentration. NB induces chromosome aberration of Vicia faba primary root cells. Single bridge, double bridge, chromosome fragment, abnormal action chromosome, syncytium and others aberration were observed. Chromosome aberration frequency at 5mg/L and higher show actual statistical significance (P<0.01) compared with the control. The significantly increasing of the MN and chromosomal aberrations frequency indicated the genotoxicity of NB on Vicia faba root tips.
机译:通过蚕豆微核(MN)测定,染色体畸变测定和有丝分裂指数测定来评价硝基苯的遗传毒性。 NB浓度从5 mg / L增加到50 mg / L后,蚕豆原根尖细胞有丝分裂指数(MI)升高。这说明NB可能影响蚕豆根尖细胞的有丝分裂,在较低浓度下促进,在较高浓度下抑制。 NB诱导蚕豆原根细胞的染色体畸变。观察到单桥,双桥,染色体片段,异常动作染色体,合胞体和其他畸变。与对照组相比,5mg / L及更高的染色体畸变频率显示出实际的统计学意义(P <0.01)。 MN和染色体畸变频率的显着增加表明NB对蚕豆根尖的遗传毒性。

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