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首页> 外文会议>2011 International Conference on Computer Distributed Control and Intelligent Environmental Monitoring >Effects of Surfactants Tween 80 and Rhamnolipid on the Extracellular Enzymes Amylase, Protease, CMCase and Xylanase of One Strain
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Effects of Surfactants Tween 80 and Rhamnolipid on the Extracellular Enzymes Amylase, Protease, CMCase and Xylanase of One Strain

机译：表面活性剂吐温80和鼠李糖脂对一种菌株胞外酶淀粉酶，蛋白酶，CMCase和木聚糖酶的影响

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摘要

The chemical surfactant Tween 80 and biosurfactantrhamnolipid were added to the solid-state fermentation processes of a strain of actinomycete Streptomyces badius. The extra cellular amylase, protease, carboxy methyl cellulose enzyme (CMCase) and xylanase were analyzed during the fermentation process. In addition, the pH of the fermentation substrate, microbial population and the degradation of the organic matter were determined. The results showed that Tween 80 increased the activities of amylase, protease, CMCase and xylanase to different extents while rhamnolipidonly had an obvious stimulatory effect on xylanase. Neither Tween 80 nor rhamnolipid had an obvious effect on the pH of the fermentation substrate. As a result of the improved enzyme activities, the degradation rates of organic matter of the treatments with surfactants were higher than that of the control. The results indicated that Tween 80 is a common stimulant for most enzymes, while rhamnolipid can only act with some specific enzyme, such as xylanase.
机译：化学表面活性剂吐温80和生物表面活性剂鼠李糖脂被添加到放线菌链霉菌菌株的固态发酵过程中。在发酵过程中分析了细胞外淀粉酶，蛋白酶，羧甲基纤维素酶（CMCase）和木聚糖酶。另外，测定了发酵底物的pH，微生物种群和有机物的降解。结果表明，吐温80可不同程度地增加淀粉酶，蛋白酶，CMCase和木聚糖酶的活性，而鼠李糖脂仅对木聚糖酶具有明显的刺激作用。吐温80和鼠李糖脂均未对发酵底物的pH产生明显影响。由于提高了酶活性，用表面活性剂处理的有机物的降解率高于对照。结果表明，吐温80是大多数酶的常见刺激剂，而鼠李糖脂只能与某些特定的酶（例如木聚糖酶）一起起作用。

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