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首页> 外文会议>2011 International Conference on Computer Distributed Control and Intelligent Environmental Monitoring >Synthesis and Identification of Artificial Antigens for an Antibody of Anthracene
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Synthesis and Identification of Artificial Antigens for an Antibody of Anthracene

机译：蒽抗体人工抗原的合成与鉴定

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To detect the residue of polycyclic aromatic hydrocarbons (PAHs)in the environment quickly by immune method, preparation of high quality antibody against PAHs is the most critical step of the project. As the first step toward developing sensitive enzyme linked immunosorbent assays (ELISAs) for selective detection of anthracene in environment, a new hapten was synthesized by the Friedel-Crafts acylation reaction from anthracene. It bears a carboxylic group with four carbon length spacer at the position 1of anthracene. The hapten was characterized by 1H NMR, IR, and element analysis. The hapten was coupled with bovine serum albumin (BSA) to raise an antibody or with ovalbumin (OVA) to form competitor conjugate. The artificial antigents were verified by their spectral characteristics using ultra violets pectrophotometry. The resultant immunogen was fabricated and used to stimulate immune responses in rabbits to survey the characteristics of the hapten. The obtained antiserum exhibited relatively high antibody titer (1:110000) in indirect ELISA.
机译：为了通过免疫方法快速检测环境中的多环芳烃（PAHs）残留，制备抗PAHs的高质量抗体是该项目最关键的步骤。作为开发可选择性检测环境中蒽的灵敏酶联免疫吸附法（ELISA）的第一步，Friedel-Crafts酰化反应由蒽合成了一种新的半抗原。它在蒽的位置1带有一个带有四个碳长度间隔基的羧基。通过1 H NMR，IR和元素分析对半抗原进行表征。将半抗原与牛血清白蛋白（BSA）偶联以产生抗体，或与卵清蛋白（OVA）偶联以形成竞争物偶联物。使用紫外线分光光度法通过光谱特征验证了人工抗原。制造了所得的免疫原，并将其用于刺激兔的免疫反应以调查半抗原的特征。在间接ELISA中，获得的抗血清表现出相对较高的抗体滴度（1：110000）。

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