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首页> 外文会议>2011 International Conference on Computer Distributed Control and Intelligent Environmental Monitoring >Isolation of High-Efficient Low-Temperature Methanogen and Comparation on Flora in Anaerobic Fermentation Production Biogas
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Isolation of High-Efficient Low-Temperature Methanogen and Comparation on Flora in Anaerobic Fermentation Production Biogas

机译：厌氧发酵生产沼气中高效低温产甲烷菌的分离及菌群比较

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With the Hun gate anaerobic techniques, a low-temperature methanogen strain was isolated from sludge in De Qing Alpine region from Tibet, named XZF21. The colony is white, not transparent, Gram-negative, uses formate as the substrate forCH4 formation, the most suitable growth temperature is 23¡æ, most suitable pH is 7.0 with yeast extract, the shape, physical characteristics and the analysis of 16S rDNA indicated that this bacteria is Methano corpusculum parvum. Methane bacteria and coupled bacteria as inoculum in the fish pond sludge and fresh pig manure, under the strict fermentation temperature (23¡À1¡æ) to contrast the biogas production. The result showed: Compared the sludge and pig manure as fermentation medium, increasing the bacterias can largely enhance the methane production. Further more, with the pig manure as fermentation matrix is more advantageous for the methane synthesis than that sludge, and the production of biogas is four times higher than the sludge, the degeneration rate of TS and VS improved25.20%-32.35%, 6.28%-14.85% respectively.
机译：使用匈奴门厌氧技术，从西藏德清高山地区的污泥中分离出低温产甲烷菌菌株，命名为XZF21。菌落为白色，不透明，革兰氏阴性，以甲酸盐为底物形成CH4，最适生长温度为23℃，最适pH为7.0，经酵母抽提，形态，物理特性及16S rDNA分析表明该细菌是小食甲烷菌（Methano corpusculum parvum）。在严格的发酵温度（23±1℃）下，鱼池污泥和新鲜猪粪中的甲烷菌和耦合菌作为接种物，以对比沼气的产生。结果表明：与污泥和猪粪作发酵培养基相比，增加细菌数量可以大大提高甲烷的产生量。此外，以猪粪为发酵基质比污泥​​更有利于甲烷合成，沼气产量是污泥的四倍，TS和VS的降解率提高了25.20％-32.35％，6.28。 ％-14.85％。

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