Cloning and function identification of the promoters and terminator of sugar beet chloroplast gene psbA

机译：甜菜叶绿体基因psbA启动子和终止子的克隆及功能鉴定

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Using two pairs of specific primers, DNA fragments containing the promoter PpsbA of psbA gene and terminator TpsbA of psbA gene, were obtained by PCR from Beta vulgaris. L chloroplast genomic DNA. Sequence analyzing results showed that the functional domains such as −35 domain, −10 domain and the translation control sequence motif in the cloned promoters the highly conserved to Nicotiana tabacum. The promoter PpsbA and the terminator TpsbA were cloned and the prokaryotic expression vectors pSKPTA of aadA gene were constructed by using PpsbA and the terminator TpsbA. Prokaryotic expression results showed that the promoters PpsbA cloned were functional and their activities could be controlled by the RNA polymerase of E.coli BL21 and the aadA controlled by them could be constitutively expressed E.coli in BL21.

机译：使用两对特定引物，通过PCR从Veta寻常中得到含有PSBA基因的PSBA基因启动子PPSBA的DNA片段和PSBA基因的终止剂TPSBA。 l叶绿体基因组DNA。序列分析结果表明，克隆的启动子中的功能域，例如-35结构域，-10结构域和平移控制序列基序是高度保守的尼古利氏菌簇。克隆促进剂PPSBA和终止子TPSBA，通过使用PPSBA和终止剂TPSBA构建AADA基因的原核表达载体pskpta。原核表达结果表明，克隆的启动子PPSBA是官能的，并且它们的活性可以由大肠杆菌BL21的RNA聚合酶控制，并且通过它们控制的AADA可以在BL21中构成大肠杆菌。

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《2011 International Conference on Remote Sensing, Environment and Transportation Engineering》|2011年|p.6679-6681|共3页
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Cui Jie; Wenjing Li; Dayou Cheng; Xin Yang; Li Binsheng;
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中图分类环境遥感;
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Function Identification; Sugar beet; chloroplast gene psbA; promoter; terminator;

机译：功能鉴定;甜菜;叶绿体基因psbA;启动子;终止子;

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机译：甜菜孢囊线虫感染后上调的基因的克隆和功能分析
2. Cloning and functional analyses of a gene from sugar beet up-regulated upon cyst nematode infection [J] . Samuelian S, Kleine M, Ruyter-Spira CP, Plant Molecular Biology . 2004,第1期

机译：甜菜孢囊线虫感染后上调的基因的克隆和功能分析
3. Identification and structure of six members of the hexokinase gene family in Vitis vinifera: Cloning, expression, and functional analysis of a putative chloroplast stromal-type hexokinase [J] . Cakir B The Journal of Horticultural Science & Biotechnology . 2014,第6期

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4. Cloning and Function Identification of the Promoters and Terminator of Sugar beet Chloroplast Gene psbA [C] . Jie Cui, Wenjing Li, Dayou Cheng, International Conference on Remote Sensing, Environment and Transportation Engineering . 2011

机译：糖束叶绿体基因PSBA启动子和终止子的克隆和功能鉴定
5. Identification, characterization, and cloning of Arabidopsis rhd1-4: A UDP-glucose-4-epimerase mutant that exhibits hypersusceptibility to the sugar beet cyst nematode and altered root ethylene responses. [D] . Wubben, Martin John Evers, II. 2004

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6. A sugar beet chlorophyll a/b binding protein promoter void of G-box like elements confers strong and leaf specific reporter gene expression in transgenic sugar beet [O] . Dietmar J Stahl, Dorothee U Kloos, Reinhard Hehl 2004

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7. A functional promoter shift of a chloroplast gene: a transcriptional fusion between a novel psbA gene copy and the trnK (UUU) gene in Pinus contorta. [O] . J Lidholm, P Gustafsson 1992

机译：叶绿体基因的功能启动子偏移：新型PSBA基因拷贝和Pinus Contorta中的TrnK（UUU）基因之间的转录融合。

Cloning and function identification of the promoters and terminator of sugar beet chloroplast gene psbA

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