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首页> 外文会议>2011 International Conference on Remote Sensing, Environment and Transportation Engineering >Selection of Streptomyces roseosporus strain with high yield by the inactivated parental strain protoplasts fusion
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Selection of Streptomyces roseosporus strain with high yield by the inactivated parental strain protoplasts fusion

机译：灭活亲本原生质体融合技术选育高产玫瑰孢链霉菌

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[Objective] To select the daptomycin-producing strain with high yield. [Method] The protoplast of Streptomyces roseosporus SDDT57 was killed by ultraviolet (Wavelength 253 nm, irradiation distance 30 cm) for 8 min, and that of Streptomyces roseosporus SUDT57 was killed by heating (75°C) for 60 min. Then both the killed protoplasts were fused in PEG. The strain with high yield was isolated from the fused protoplasts. [Result] The strain with the highest yield was named S. roseosporus SF06. The Shake flask fermentation was performed with capric acid. The production was improved to 113.74 mg/L, which was 2.46 and 2.47 times that of the two original strains respectively. [Conclusion] It is viable to select daptomycin-producing strain with high yield by the inactivated parental strain protoplasts fusion. This study provides much for the next research on Genome shuffling of Streptomyces roseosporus.
机译：［目的］选择高产达托霉素的菌株。 [方法]紫外线（波长253 nm，照射距离30 cm）处理8分钟，对玫瑰链霉菌SDDT57的原生质体进行加热;在75°C加热60分钟，对玫瑰链霉菌SUDT57的原生质体进行杀死。然后将两种杀死的原生质体都融合在PEG中。从融合的原生质体中分离出高产菌株。 [结果]将产量最高的菌株命名为玫瑰孢孢SF6。摇瓶发酵用癸酸进行。产量提高到113.74 mg / L，分别是两个原始菌株的2.46和2.47倍。 [结论]通过灭活的亲本菌株原生质体融合选择高产达托霉素的菌株是可行的。该研究为玫瑰孢链霉菌的基因组改组的下一步研究提供了很多。

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