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首页> 外文会议>2011 International Conference on Remote Sensing, Environment and Transportation Engineering >Optimization of ultrasound-assisted extraction of malania oleifera chum polysaccharides using response surface methodology and its antioxidative effect
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Optimization of ultrasound-assisted extraction of malania oleifera chum polysaccharides using response surface methodology and its antioxidative effect

机译：响应面法优化超声波辅助提取香叶多糖的工艺及其抗氧化作用

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Response surface methodology(RSM) was used for optimizing ultrasound-assisted extraction of malania polysaccharides. Based on single-factor experiments, three independent variables, namely extraction time(A), ultrasonic temperature(B), and material/water ratio(C), were selected as casual factors. The experiments were arranged according to Box-Behnken central composite experiment design. Response surface methodology was applied to determine the effect of central composite experiment design and casual factors on the yield of malania polysaccharides(Y) which were determined by the phenol-sulfuric acid method. In this foundation, Design Expert software was employed and a second order quadratic equation for malania polysaccharides extraction was established: Y=4.29+0.16A+0.18B+0.39C−0.080AB+0.072AC+0.23BC−0.45A2+0.21B2−0.15C2. It showed that the factors of affecting the extraction rate of malania polysaccharides in order were as fo1lows: material/water ratio(C)>ultrasonic temperature(B)> extraction time(A). The model equation was very significant (P<0.0001), and adjustment R2 was 0.9792. It demonstrated that the degree of model fitting was well. By analyzing the response surface plots and their corresponding contour plots as well as solving the quadratic equation, the optimal values of the variables were as follows: extraction time 45 min, ultrasonic temperature 36.67 °C, and material/water ratio 1∶25 (g/mL). Under such conditions, the predicted best extraction yield of malania polysaccharides was 5.17%, which was very consistent with the experimental value (5.02%) with a relative error of 2.90%. The applicability of the model equation for predicting the optimum response values was verified effectively by the validation data. Antioxidant biological activity of malania polysaccharides was studied under two different situations. The result showed that the inhibition-- rates of malania polysaccharides separated by ceramic membrane microfiltration and alcohol sedimentation on hydroxyl free radical and DPPH free radical were increased significantly within the range of certain concentrations. The highest hydroxyl free radical and DPPH free radical scavenging activity (73.18% and 77.59%) were found with 4.57 mg/mL and 2.44 mg/mL, respectively. It indicated that malania polysaccharides could be as new, safe, effective and natural antioxidants.
机译：响应面法（RSM）被用于优化超声辅助提取疟疾多糖。在单因素实验的基础上，选择了提取时间（A），超声温度（B）和物水比（C）这三个独立变量作为偶然因子。实验根据Box-Behnken中央复合实验设计进行安排。应用响应面分析法确定了中心复合实验设计和偶然因素对酚-硫酸法测得的Malania多糖（Y）收率的影响。在此基础上，使用Design Expert软件，建立了用于提取疟疾多糖的二次方程式：Y = 4.29 + 0.16A + 0.18B + 0.39C-0.080AB + 0.072AC + 0.23BC-0.45A 2 + 0.21B 2 −0.15C 2 。结果表明，影响丹参多糖提取率的因素依次为：物料/水比（C）>超声波温度（B）>提取时间（A）。模型方程非常显着（P <0.0001），调整量R 2 为0.9792。表明模型拟合程度良好。通过分析响应面图及其对应的等高线图并求解二次方程，变量的最佳值如下：提取时间为45分钟，超声温度为36.67°C，料水比为1∶25（g / mL）。在这种条件下，预测的疟原虫多糖的最佳提取率为5.17％，与实验值（5.02％）非常一致，相对误差为2.90％。验证数据有效地验证了模型方程式预测最佳响应值的适用性。在两种不同情况下研究了疟原虫多糖的抗氧化生物学活性。结果表明，抑制作用- -- 在一定浓度范围内，通过陶瓷膜微滤和乙醇沉降的羟基自由基和DPPH自由基分离出的疟疾多糖的比率显着增加。发现最高的羟基自由基清除能力和DPPH自由基清除活性分别为4.57 mg / mL和2.44 mg / mL，分别为73.18％和77.59％。这表明疟疾多糖可以作为新型，安全，有效和天然的抗氧化剂。

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