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首页> 外文会议>NSTI nanotechnology conference and expo;Nanotech conference expo;NSTI-Nanotech 2012;TechConnect world annual conference expo >Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without adsorption
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Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without adsorption

机译：疏水性有机硅弹性体腔室，用于记录运动性​​猪精子的运动而无需吸附

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Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P < 0.05) and ALHD (P < 0.05) in motile porcine sperm on glass preparations compared with those on PDMS preparations. These differences were due to adsorption of the head and/or neck to hydrophilic substrates. Because of the elasticity of PDMS, we propose that a PDMS membrane should be used for CASA. To investigate the dynamics of motile porcine sperms with microfluidics, we do not recommend plasma treatment to bond PDMS and glass in the microchannel preparation; instead, we suggest that a PDMS molding process without plasma treatment be used for preparation of microfluidic channels.
机译：运动的猪精子粘附在亲水性材料上，例如玻璃和塑料。与玻璃相比，精子对疏水性聚二甲基硅氧烷（PDMS）膜的吸附较少。我们使用计算机辅助精子分析（CASA）研究了玻璃和PDMS制剂上运动的猪精子的线速度（LV）和横向头部位移（ALHD）的幅度。与PDMS制剂相比，玻璃制剂中运动型猪精子的15分钟LV（P <0.05）和ALHD（P <0.05）显着降低。这些差异是由于头部和/或颈部吸附在亲水性基材上。由于PDMS的弹性，我们建议将PDMS膜用于CASA。为了研究带有微流控技术的运动型猪精子的动力学，我们不建议在微通道制备中进行血浆处理以使PDMS和玻璃结合。相反，我们建议使用未经等离子体处理的PDMS成型工艺来制备微流体通道。

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