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首页> 外文会议>International Symposium on Information Technologies in Medicine and Education;ITME 2012 >Preparation and biological performance evaluation of Lactoferrin/ Nanosized hydroxyapatite/ Collagen I composite material
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Preparation and biological performance evaluation of Lactoferrin/ Nanosized hydroxyapatite/ Collagen I composite material

机译：乳铁蛋白/纳米羟基磷灰石/ I型胶原复合材料的制备及生物学性能评价

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Objective: To prepare and analyse Lactoferrin/ Nanosized hydroxyapatite/ Collagen I(CMs-loaded LFHA/Co-I) composite materials. Methods: Tripolyphosphate (TPP) was used as cross-linker to make CMs-loaded LF. nHA was prepared through Chemical precipitation. Collagen I(Co- I) was extracted from fresh bovine tendon through acid dissolution and protease digestion.CMs, nHA and collagen could be crosslinked by Genipin to be tissue engineering materials.The composite materials would be tested by scanning electron microscopy(SEM), enzyme-linked immuno sorbent assay(ELISA) immunohistochemistry, X-ray analysis, infrared spectroscopy, average bone density X-ray and analysis by computer software.Results: Genipin could effectively crosslink CNs, nHA and collagen together to form engineering material to repair bone defect.
机译：目的：制备和分析乳铁蛋白/纳米羟基磷灰石/胶原蛋白I（CMs负载的LF / nHA / Co-I）复合材料。方法：以三聚磷酸酯（TPP）为交联剂制备负载CMs的LF。通过化学沉淀制备nHA。通过酸溶解和蛋白酶消化从新鲜牛腱中提取胶原I（Co-I），CM，nHA和胶原可通过Genipin交联成为组织工程材料，复合材料将通过扫描电子显微镜（SEM）进行测试，酶联免疫吸附测定（ELISA）免疫组化，X射线分析，红外光谱，平均骨密度X射线和计算机软件分析。结果：Genipin可以有效地将CNs，nHA和胶原蛋白交联在一起，形成修复骨骼的工程材料。缺点。

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