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首页> 外文会议>International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale >Investigating the relationship between CD20-Rituximab binding force and mechanical properties of Lymphom B cells using atomic force microscopy
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Investigating the relationship between CD20-Rituximab binding force and mechanical properties of Lymphom B cells using atomic force microscopy

机译：使用原子力显微镜研究CD20-利妥昔单抗结合力与淋巴B细胞力学性质之间的关系

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The invention of atomic force microscopy (AFM) provides new technology for investigating the physiological activities at the single cell and single molecule levels. Lymphoma Raji cells were adsorbed onto the glass slides by coating the glass slides with poly-L-lysine. Rituximabs (anti-CD20 antibody) were linked onto the AFM tip by PEG linker and the CD20-Rituximab binding forces were measured on three lymphoma Raji cells. Then the mechanical properties of the Raji cells were dynamically measured after the Rituximab activation by obtaining force curves on the cell surface. The mechanical properties of lymphoma cells kept stable at the first 20–30 min, and then decreased markedly. Besides, as the CD20-Rituximab binding force increased, the mechanical properties decrease rate increased. The experimental results indicated that the Rituximab's effect is related to CD20-Rituximab binding force and can improve our understanding of Rituximab's variable efficacies in different patients.
机译：原子力显微镜（AFM）的发明为研究单细胞和单分子水平的生理活性提供了新技术。通过用聚-L-赖氨酸涂覆载玻片，将淋巴瘤Raji细胞吸附到载玻片上。通过PEG接头将利妥昔单抗（抗CD20抗体）连接到AFM尖端上，并在三种淋巴瘤Raji细胞上测量了CD20-利妥昔单抗结合力。然后在利妥昔单抗激活后通过获得细胞表面的力曲线来动态测量Raji细胞的机械性能。淋巴瘤细胞的机械性能在最初的20-30分钟内保持稳定，然后显着下降。此外，随着CD20-利妥昔单抗结合力的增加，机械性能下降速率增加。实验结果表明，利妥昔单抗的作用与CD20-利妥昔单抗的结合力有关，可以提高我们对利妥昔单抗在不同患者中疗效的了解。

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