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首页> 外文会议>IEEE International Conference on Industrial Engineering and Engineering Management >Laboratory measurement: Chlorophyll-a concentration measurement with acetone method using spectrophotometer
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Laboratory measurement: Chlorophyll-a concentration measurement with acetone method using spectrophotometer

机译：实验室测量：使用分光光度计使用分光光度计与丙酮方法的浓度测量

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Chlorophyll-a is an important factor in photosynthesis of microalgae. The concentration of chlorophyll-a in microalgae can be used to analyse and investigate the density and biomass of microalgae in the ecosystem. This is because microalgae are primary producer in the food web in freshwater and ocean. Thus, an accurate estimate of chlorophyll-a concentration in microalgae is very significant. In this study, the samples of microalgae were collected from Tasik Harapan and Tasik Aman, USM Penang, Malaysia. The samples were tested using 90% acetone method by using spectrophotometer (model HITACHI U-1900) to record the absorbance values with selected wavelengths namely 750 nm, 664 nm, 647 nm, and 630 nm The results showed that the chlorophyll-a concentration in microalgae increased when the absorbance was increased. The chlorophyll-a concentration in microalgae depended on several factors such as amount of sunlight available, total phosphorus (TP), water flow, light, catchment area, water depth, weather, and other physical factors. This study determined the value of chlorophyll-a concentration in microalgae collected from Tasik Harapan and Tasik Aman, USM Penang. The highest and the lowest chlorophyll-a concentration were recorded in this study. The spectrophotometer with 90% acetone method successfully determined the maximum and minimum sensitivity of chlorophyll-a concentration within the wavelengths tested.
机译：叶绿素-A是微藻光合作用的一个重要因素。微藻中叶绿素-A的浓度可用于分析和研究生态系统中微藻的密度和生物量。这是因为微藻是在淡水和海洋中食品网中的主要生产者。因此，微藻中叶绿素-A浓度的准确估计非常显着。在这项研究中，从马来西亚槟榔岛的Tasik Harapan和Tasik Aman收集了微藻样品。通过使用分光光度计（Mathachi U-1900）使用90％丙酮方法测试样品，以使所选波长的吸光度值纳入750nm，664nm，647nm和630nm，结果显示叶绿素 - a浓度当吸光度增加时，微藻增加。微藻中的叶绿素 - 一种浓度依赖于可用的阳光量，总磷（TP），水流，光，集水区，水深，天气和其他物理因素等几个因素。这项研究确定了叶绿素-A浓度的微藻浓度从Tasik Harapan和Tasik Aman，USM槟城。在本研究中记录了最高和最低的叶绿素-A浓度。具有90％丙酮方法的分光光度计成功地确定了经过测试的波长内叶绿素-A浓度的最大和最小敏感性。

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