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首页> 外文会议>Conference on photonic therapeutics and diagnostics IX >Non-invasive imaging of prostate cancer progression in nude mice using iRFP gene reporter
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Non-invasive imaging of prostate cancer progression in nude mice using iRFP gene reporter

机译：使用iRFP基因报告子对裸鼠前列腺癌进展进行非侵入性成像

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Prostate cancer (PCa) is the second most common cancer in US men. Metastasis is the final step of tumor progression and remains the primary cause of PCa death. Hence preclinical, orthotopic models of PCa metastasis are necessary to develop new therapeutics against metastatic disease. Yet unlike irrelevant subcutaneous tumor models, the deployment of orthotopic models of cancer metastasis in drug research and development is limited by the inability to longitudinally monitor cancer progression/regression in response to administration of experimental Pharmaceuticals. Recently, a near-infrared fluorescent protein (iRFP) was created for deeper imaging [1]. Imaging prostate tumor growth and lymph node metastasis in nude mice therefore becomes possible using this new fluorescent gene reporter. In this study, we first developed an intensified CCD (ICCD)-based iRFP fluorescence imaging device. Then human PCa PC3 cell lines expressing iRFP gene reporter were orthotopically implanted in male Nu/Nu mice at 8-10 weeks old. After 6-10 weeks, in vivo, in situ and ex vivo fluorescence imaging was performed. In vivo iRFP fluorescence imaging showed that the detected fluorescence concentrated at the prostate and became stronger over time, indicating the growth of implanted PCa. Fluorescence was non-invasively detected at locations of prostate-draining lymph nodes as early as 5 weeks post implantation, indicating the metastasis to lymph nodes. In situ and ex vivo fluorescence imaging demonstrated that the detected signals from PCa and lymph nodes were correlated with cancer positive status of tissues as assessed through standard pathology.
机译：前列腺癌（PCa）是美国男性中第二常见的癌症。转移是肿瘤进展的最后一步，仍然是PCa死亡的主要原因。因此，PCa转移的临床前，原位模型对于开发针对转移性疾病的新疗法是必不可少的。然而，与无关的皮下肿瘤模型不同，在药物研究和开发中原位癌转移模型的部署受到无法纵向监测癌症进展/消退以响应实验药物给药的限制。最近，近红外荧光蛋白（iRFP）被创建用于更深的成像[1]。因此，使用这种新的荧光基因报道分子可以在裸鼠中对前列腺肿瘤的生长和淋巴结转移进行成像。在这项研究中，我们首先开发了基于增强CCD（ICCD）的iRFP荧光成像设备。然后将表达iRFP基因报告基因的人PCa PC3细胞系原位植入8-10周龄的雄性Nu / Nu小鼠中。 6-10周后，进行体内，原位和离体荧光成像。体内iRFP荧光成像显示检测到的荧光集中在前列腺上，并随着时间的推移变得更强，表明植入的PCa的生长。早在植入后5周就在前列腺引流淋巴结的位置无创检测到荧光，表明转移至淋巴结。原位和离体荧光成像显示，通过标准病理学评估，从PCa和淋巴结中检测到的信号与组织的癌阳性状态相关。

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