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Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces

机译：使用共价固定的捕获寡核苷酸探针在玻璃和聚二甲基硅氧烷表面上检测有害藻华的微藻

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Harmful algal bloom (HAB) events have been on the rise in the last few decades with some of the causative microalgae exhibiting toxic properties. Therefore, detection is essential in order to prevent mortality of aquatic life and poisoning events from consumption of these biotoxins. Here, oligonucleotide modified glass and poly(dimethylsiloxane) (PDMS) surfaces have been developed for the detection of the HAB causing microalgae, Alexandrium catenella, in a model system. Our preliminary studies show that the glass surface offers superior stability and analytical response when compared to those prepared from PDMS.

机译：在过去的几十年中，有害的藻华（HAB）事件一直在增加，某些致病性微藻具有毒性。因此，为了防止由于消耗这些生物毒素而导致水生生物死亡和中毒事件，检测是必不可少的。此处，已开发出寡核苷酸修饰的玻璃和聚二甲基硅氧烷（PDMS）表面，用于在模型系统中检测引起微藻的HAB，即亚历山大连叶藻。我们的初步研究表明，与由PDMS制备的玻璃相比，玻璃表面具有出色的稳定性和分析响应。

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《Conference on microano materials, devices, and systems》|2013年|89235B.1-89235B.10|共10页
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Karen L. Bruce; Amanda V. Ellis; Sophie C. Leterme; Dmitriy A. Khodakov; Claire E. Lenehan;
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Harmful algal bloom (HAB); hybridisation; surface modification; covalent attachment; Alexandrium catenella;

机译：有害藻华（HAB）;杂交表面改性;共价连接亚历山大草;

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2. The state of U.S. freshwater harmful algal blooms assessments, policy and legislation. (Special Issue: Harmful algal blooms and natural toxins in fresh and marine waters - exposure, occurrence, detection, toxicity, control, management and policy.) [J] . Hudnell H. K. Toxicon: An International Journal Devoted to the Exchange of Knowledge on the Poisons Derived from Animals, Plants and Microorganisms . 2010,第5期

机译：美国淡水有害藻华状况评估，政策和法规。（特刊：淡水和海水中有害的藻华和天然毒素-暴露，发生，检测，毒性，控制，管理和政策。）
3. Erratum to: Evaluation of locked nucleic acids for signal enhancement of oligonucleotide probes for microalgae immobilised on solid surfaces [J] . Sonja Diercks, Christine Gescher, Katja Metfies, Journal of Applied Phycology . 2010,第6期

机译：勘误到：评估锁定核酸以增强寡核苷酸探针固定在固体表面上的微藻的信号增强
4. Development of coastal upwelling edge detection algorithms associated with harmful algal blooms off the Washington coast using sea surface temperature imagery [C] . Nathan R. Evans, Dana L. Woodruff, Vera L. Trainer Remote Sensing of the Coastal Oceanic Environment . 2005

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5. Differentiation of morphology and toxicity in harmful algal blooms caused by the raphidophyte alga Heterosigma akashiwo. [D] . Black, Edward Adam. 2000

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6. Molecular detection and species identification ofAlexandrium (Dinophyceae) causing harmful algal blooms along theChilean coastline [O] . Ana Jedlicki, Gonzalo Fernández, Marcela Astorga, -1

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7. Molecular detection and species identification of Alexandrium (Dinophyceae) causing harmful algal blooms along the Chilean coastline [O] . Ana Jedlicki, Gonzalo Fernández, Marcela Astorga, 2012

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8. Real-Time Monitoring for Toxicity Caused by Harmful Algal Blooms and Other Water Qualtiy Perturbations. [R] . 2001

机译：实时监测有害藻类和其他水质扰动引起的毒性。

Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces

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