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Green synthesis of silver nanoparticles as an antibacterial agent using Rhodomyrtus tomentosa acetone extract

机译:毛绒球菌丙酮提取物绿色合成纳米银作为抗菌剂

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The capability of Rhodomyrtus tomentosa acetone extract (RAE) for the production of silver nanoparticles (AgNPs) has been explored for the first time. Silver nanoparticles with a surface plasmon resonance band centered at 420-430 nm were synthesized by reacting RAE with AgNO_3. Reaction time, temperature, concentration of AgNO3 and RAE could accelerate the reduction rate of Ag~+ and affect AgNPs size. The nanoparticles were found to be 10-30 nm in size and spherical in shape. XRD data demonstrated crystalline nature of AgNPs dominated by (200) facets. FTIR results showed decrease in intensity of peaks at 3394, 1716 and 1618 cm~(-1) indicating the involvement of O-H, carbonyl group and C=C stretching with the formation of AgNPs with RAE, respectively. The C-O-C and C-N stretching suggested the presence of many phytochemicals on the surface of the nanoparticles. High negative zeta potential values confirmed the stability of AgNPs in water. In vitro antibacterial activity of AgNPs was tested against Staphylococcus aureus using broth microdilution method. AgNPs capped with RAE demonstrated profound antibacterial activity against the organisms with minimum inhibitory concentration and minimum bactericidal concentration in the range between 3.1-6.2 and 6.2-50 μgmL~(-1), respectively. The synthesized nanoparticles could be applied as an effective antimicrobial agent against staphylococcal infections.
机译:首次探究了毛绒球菌丙酮提取物(RAE)生产银纳米颗粒(AgNPs)的能力。通过使RAE与AgNO_3反应,合成了以420-430 nm为中心的表面等离振子共振带的银纳米颗粒。反应时间,温度,AgNO3和RAE的浓度可以加快Ag〜+的还原速度,并影响AgNPs的大小。发现纳米颗粒的尺寸为10-30nm并且为球形。 XRD数据表明,由(200)个晶面主导的AgNP的晶体性质。 FTIR结果表明,在3394、1716和1618 cm〜(-1)处的峰强度下降,表明O-H,羰基和C = C的牵伸分别与RAE形成的AgNPs有关。 C-O-C和C-N拉伸表明纳米粒子表面上存在许多植物化学物质。高的负Zeta电位值证实了AgNP在水中的稳定性。使用肉汤微稀释法测试了AgNPs在体外对金黄色葡萄球菌的抗菌活性。用RAE封端的AgNPs表现出对细菌的强大抗菌活性,其最小抑菌浓度和最小杀菌浓度分别在3.1-6.2和6.2-50μgmL〜(-1)之间。合成的纳米粒子可作为抗葡萄球菌感染的有效抗菌剂。

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