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Isolation and Identification of Saline Tolerance Phosphate-Solubilizing Bacteria Derived from Salt-affected Soils and Their Mechanisms of P-solubilizing

机译:盐渍土中耐盐碱溶性细菌的分离鉴定及其增磷机理

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The salt-affected soils of beach from Tianjin China were sampled to screen the saline tolerance phosphate-solubilizing bacteria (SA-T-PSB) using inorganic phosphorous medium. On basis of the phenotypic characterization and 16S rRNA gene sequencing, 4 isolates with the highest PSA, B1114, B1213, B1303, and J101 were identified as Enterobacter ludwigii, Pantoea ananatis, Pseudomonas psychrotolerans, and Gluconobacter frateurii, respectively. Subsequently, aimed to assaying the mechanisms of P-solubilizing, organic acid types of the 4 isolates were determined by high performance liquid chromatography. The results showed that all 4 isolates mainly secreted gluconic acid. The effect of carbon and nitrogen source on P-solubilization activity of the strains also indicated that the production of gluconic acid is the main mechanisms of P-solubilizing.
机译:采样来自中国天津海滩的受盐影响的土壤,以使用无机磷介质筛选耐盐溶磷的细菌(SA-T-PSB)。根据表型特征和16S rRNA基因测序,分别将PSA,B1114,B1213,B1303和J101最高的4个分离株分别鉴定为路德维氏肠杆菌,菠萝泛酸杆菌,精神假单胞菌和乳果糖杆菌。随后,旨在分析P增溶的机理,通过高效液相色谱法确定了4种分离物的有机酸类型。结果显示,所有4种分离物主要分泌葡糖酸。碳源和氮源对菌株P-增溶活性的影响也表明,葡萄糖酸的产生是P-增溶的主要机理。

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