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首页> 外文会议>ASME bioengineering conference >TOWARDS A CLINICALLY APPLICABLE TISSUE ENGINEERED VASCULAR GRAFT
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TOWARDS A CLINICALLY APPLICABLE TISSUE ENGINEERED VASCULAR GRAFT

机译：走向临床适用的组织工程化血管移植

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Decellularized arterial tissue has shown promising use as a scaffold for vascular tissue replacement; similar structural and functional characteristics to the native tissue are maintained and these scaffolds are non-thrombogenic, non immunogenic with the ability to remodel and grow in vivo. However, there still remains a number of limiting factors in clinically translating these scaffolds. Namely, producing a range of geometries to accommodate a large patient cohort within clinically feasible manufacturing times and costs. Furthermore, these scaffolds must be suitable for long term preservation to produce a reasonable shelf life and be capable of undergoing standard sterilization techniques. We previously described a method to reduce manufacturing times by tailoring decellularized porcine arterial scaffolds to allow bulk cell seeding of the medial layer . This study aims to further advance this scaffold toward clinical applicability by creating a means to rapidly load the scaffold with rat mesenchymal stem cells (rMSCs) and/or therapeutics which have the ability to enhance medial cell repopulation. A thermoresponsive injectable chitosan/p-glycerophosphate (P-GP) hydrogel was used as a delivery vehicle/carrier for murine hepatocyte growth factor (HGF). HGF was used to enhance scaffold repopulation as HGF has been shown to promote cell migration . Moreover, optimum freeze-drying parameters for these scaffolds which allows long term preservation and sterilization are also described.
机译：脱细胞的动脉组织已显示出有望用作血管组织置换的支架。维持与天然组织相似的结构和功能特征，并且这些支架是非血栓形成的，非免疫原性的，具有在体内重塑和生长的能力。然而，在临床上翻译这些支架时仍然存在许多限制因素。即，产生一定范围的几何形状以在临床可行的制造时间和成本内容纳大批患者。此外，这些支架必须适合于长期保存以产生合理的保存期限，并且能够经受标准的灭菌技术。我们先前描述了一种通过定制去细胞猪动脉支架来减少制造时间的方法，以允许大量的细胞植入中间层。这项研究的目的是通过创建一种快速向支架中加载大鼠间充质干细胞（rMSCs）和/或具有增强内侧细胞重聚能力的治疗剂的方法，进一步将该支架向临床应用发展。使用热响应性壳聚糖/对-甘油磷酸酯（P-GP）水凝胶作为鼠肝细胞生长因子（HGF）的递送载体/载体。 HGF被用来增强支架的人口增长，因为HGF已被证明可以促进细胞迁移。此外，还描述了这些支架的最佳冷冻干燥参数，其可以长期保存和灭菌。

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