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首页> 外文会议>IEEE International Conference on Signal Processing, Communications and Computing >Automatic detection of comets in Silver stained comet assay images for DNA damage analysis
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Automatic detection of comets in Silver stained comet assay images for DNA damage analysis

机译：自动检测银染彗星测定图像中的彗星以进行DNA损伤分析

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摘要

Comet assay or Single Cell Gell Electrophoresis (SCGE) is a standard method for detecting Deoxyribo Nucleic Acid (DNA) damage in individual cells. The final stage of comet assay process is image acquisition and analysis. An effective automatic method for detecting comets from very noisy comet assay images is proposed in this paper. Most of the related work in this area are based on fluorescent stained comet assay images. But, silver staining method is commonly used for clinical applications in developing countries like India as it is inexpensive, slides can be preserved for a long time and is less hazardous. Currently most of the comet assay systems employ image analysis modules that are semi-automated which have the disadvantages of requirement of skilled personnel and having limited throughput. Hence, it is evident that a fully automated image analysis system is critical for rapid and accurate DNA damage detection. Therefore, an automatic comet detection stage as part of a fully automated comet assay image analysis system for DNA damage analysis and quantification is developed and implemented here. Based on our results and evaluation by a clinical expert, the performance of the proposed method is found to be better than that of the existing methods in terms of %TP, %FN, %FP, PPV and sensitivity.
机译：彗星测定法或单细胞凝胶电泳（SCGE）是检测单个细胞中脱氧核糖核酸（DNA）损伤的标准方法。彗星测定过程的最后阶段是图像采集和分析。提出了一种从噪声很大的彗星试验图像中检测彗星的有效自动方法。该领域的大多数相关工作都基于荧光染色的彗星试验图像。但是，银染法由于价格便宜，载玻片可以保存很长时间且危害较小，因此在印度等发展中国家通常用于临床。当前，大多数彗星化验系统使用半自动化的图像分析模块，其具有需要熟练技术人员并且产量有限的缺点。因此，很明显，全自动图像分析系统对于快速，准确地检测DNA损伤至关重要。因此，在此开发并实现了作为用于DNA损伤分析和定量的全自动彗星分析图像分析系统的一部分的自动彗星检测阶段。根据我们的结果和临床专家的评估，在％TP，％FN，％FP，PPV和灵敏度方面，发现该方法的性能优于现有方法。

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