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首页> 外文会议>IEEE International Conference on Industrial Technology >Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads
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Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads

机译：DNA长度对DNA标记微珠的介电泳特性的影响

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Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. We have developed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads that are chemically labeled with the amplicons. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of amplicons. The method is based on the surface conductivity dependence of microbeads DEP characteristics. It was expected that the surface conductivity would depend on the length of DNA fragments immobilized on a microbeads. In this study, it was found that the crossover frequency was dependent on the length of DNA.
机译：聚合酶链反应（PCR）是用于细菌和病毒感染诊断程序的强大工具。我们已经开发出一种新的电学技术，用于快速检测通过使用扩增子进行化学标记的微珠的介电电泳（DEP）进行PCR扩增的DNA。固定在微珠上的DNA改变了它们的DEP行为，使得它们在正DEP的作用下被捕获在微电极上，而原始的微珠则没有。将DEP特性的显着变化与阻抗测量相结合，可以快速，定量地检测扩增子。该方法基于微珠DEP特性的表面电导率依赖性。预期表面电导率将取决于固定在微珠上的DNA片段的长度。在这项研究中，发现交叉频率取决于DNA的长度。

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