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Inhibition by low-temperature plasma jet on the viability of hepatoma cells and its mechanism

机译：低温血浆射流对肝癌细胞活力的抑制作用及其机制

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Many recently studies have demonstrated that low-temperature plasma (LTP) can induce apoptosis and necrosis in a variety of carcinoma cells in vitro. However, the inhibition mechanisms of LTP on carcinoma cells have been still largely unknown. This study is aimed to investigate the inhibition effect and mechanism of LTP on hepatoma cells. An argon atmospheric pressure plasma jet is used to generate LTP for the treatment of Hep3B human hepatoma cells cultured in vitro. LTP treatment time intervals are set as 5, 10, 15, 20 and 25 s, respectively. After being treated with LTP, cell viability, cell apoptosis, cell cycle distribution and intracellular reactive oxygen species (ROS) assay of hepatoma cells are respectively measured. Experimental results show that, compared with the control cells, treatment of hepatoma cells with 15, 20 and 25 s of plasma result in a significant reduction in cell viability. Based on the exploration of related mechanisms, it is suggested that ROS generated in LTP may reduce hepatoma cell viability by inducing cell cycle arrest and cell apoptosis.
机译：最近的许多研究表明，低温血浆（LTP）可以在体外诱导多种癌细胞的凋亡和坏死。然而，LTP对癌细胞的抑制机制仍是很大程度上未知的。本研究旨在探讨LTP对肝癌细胞的抑制作用及其机制。氩气压等离子体射流用于产生LTP，以治疗体外培养的Hep3B人肝癌细胞。 LTP治疗时间间隔分别设置为5、10、15、20和25 s。用LTP处理后，分别测量肝癌细胞的细胞活力，细胞凋亡，细胞周期分布和细胞内活性氧（ROS）测定。实验结果表明，与对照细胞相比，用15、20和25 s血浆处理肝癌细胞会导致细胞活力显着降低。在探索相关机制的基础上，提示LTP中产生的ROS可能通过诱导细胞周期停滞和细胞凋亡而降低肝癌细胞的生存能力。

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