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首页> 外文会议>National SBIR/STTR conference;Annual nanotech conference and expo;Annual TechConnect world innovation conference expo >Co-Culturing Dorsal Root Ganglion Neurons with Schwann Cells Protects Them against the Cytotoxic Effects of Silver Nanoparticles
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Co-Culturing Dorsal Root Ganglion Neurons with Schwann Cells Protects Them against the Cytotoxic Effects of Silver Nanoparticles

机译：与雪旺氏细胞共同培养背根神经节神经元保护他们免受银纳米粒子的细胞毒作用。

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Because of their presumed inertness, silver nanoparticles are increasingly employed in many consumer products. Nonetheless, their environmental and health hazard impact are not understood. Our previous studies led us to develop a co-culture model consisting of dorsal root ganglion (DRG) neurons and Schwann cells and to employ it to investigate our hypothesis that co-culturing DRG neurons with Schwann cells imparts protection on them against cytotoxicity induced by silver nanoparticles. Our results indicated that silver nanoparticles induced concentration-and time-related decreases in survival of DRG neurons or Schwann cells in monotypic cultures: both cell types withstood the cytotoxicity of silver nanoparticles and survived better when maintained in co-cultures. DRG neurons and co-cultures of DRG neurons and Schwann cells, but not Schwann cells alone, expressed synapsin. Silver nanoparticles suppressed synapsin expression in DRG neurons alone but not in co-cultures with Schwann cells. Schwann cells and co-cultures of DRG neurons and Schwann cells, but not DRG neurons alone, expressed glial fibrillary acidic protein (GFAP). However, silver nanoparticles markedly suppressed GFAP expression in Schwann cells alone but not in co-cultures with DRG neurons. Thus, our results provide support for our hypothesis and may be relevant to toxicological studies prior to clinical trials of drugs formulated with agents containing silver nanoparticles.
机译：由于它们的惰性，因此银纳米颗粒越来越多地用于许多消费产品中。但是，它们的环境和健康危害影响尚不明确。我们以前的研究使我们建立了由背根神经节（DRG）神经元和雪旺氏细胞组成的共培养模型，并用它来研究我们的假设：将DRG神经元与雪旺氏细胞共培养可以保护它们免受银诱导的细胞毒性纳米粒子。我们的结果表明，银纳米颗粒在单一型培养物中诱导了DRG神经元或雪旺氏细胞的存活率与浓度和时间相关的降低：两种细胞类型均能抵抗银纳米颗粒的细胞毒性，并且在共培养时能更好地存活。 DRG神经元和DRG神经元与Schwann细胞的共培养物，而不是单独的Schwann细胞，均表达突触蛋白。银纳米颗粒仅在DRG神经元中抑制突触蛋白表达，但在与雪旺氏细胞共培养时不能抑制突触蛋白表达。雪旺氏细胞以及DRG神经元和雪旺氏细胞的共培养物表达神经胶质原纤维酸性蛋白（GFAP），但不单独表达DRG神经元。然而，银纳米颗粒显着抑制雪旺细胞中的GFAP表达，而与DRG神经元共培养时则不能。因此，我们的结果为我们的假设提供了支持，并且可能与用含银纳米粒子的制剂配制的药物的临床试验之前的毒理学研究有关。

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