• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>National SBIR/STTR conference;Annual nanotech conference and expo;Annual TechConnect world innovation conference expo >Cellular Uptake Behavior of Glycoconjugated and Micellar-Encapsulated Quantum Dots on HeLa Cervical Cancer Cells
【24h】

Cellular Uptake Behavior of Glycoconjugated and Micellar-Encapsulated Quantum Dots on HeLa Cervical Cancer Cells

机译：糖共轭和胶束包裹的量子点对HeLa宫颈癌细胞的细胞摄取行为

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glyconanomaterials are of great interest for bio-imaging to understand biological processes, for instance metabolism, cell-cell or virus-cell interactions, gluconeogenesis, and cancer. Herein, we present a strategy for the biofunctionalization of fluorescent quantum dots (QDs) from continuous-flow reactor with carbohydrates. This flow reactor enables the reproducible synthesis of a large amount of QDs, with controlled surface functionalization. These QDs act as fluorescent biomarkers and as structural scaffolds for the presentation of glycoclusters to lectins, receptors and cells. Before the phase transfer into water takes place, the carbohydrates are covalently attached to an amphiphilic poly(isoprene)-b-poly(ethylene glycol) diblock copolymer (PI-b-PEG) using click-chemistry as previously described by us. These functionalized polymers are subsequently used for the encapsulation of the QDs under preservation of their unique optical properties in a continuous flow system. Binding of glycoconjugated QDs to the human cervical cancer cell line HeLa was characterized using confocal microscopy. Depending on the terminal group of the polymer (namely: D-maltose, D-glucose, carboxyl, and amine), the uptake of the functionalized QDs can be controlled and directed. Functionalization with maltose yields very high uptake in low incubation times and low concentrations. Although serum is known to inhibit the cellular response of artificial nanostructures, we observe reduced but significant cellular uptake of the maltose functionalized nanocontainers in serum containing media. This encapsulated materials have already been tested to be suitable for in vivo tumor targeting, due to their lack of toxicity as well as extraordinary stability. Though this method relies on highly reproducible continuous flow systems, which yield high amounts of well defined, functional, non-toxic and highly stable nanoparticles this method has extraordinary industrial, biological and medical relevance.
机译：糖基纳米材料对于生物成像以了解生物学过程（例如新陈代谢，细胞-细胞或病毒-细胞相互作用，糖异生和癌症）具有极大的兴趣。在这里，我们提出了从连续流反应器与碳水化合物的荧光量子点（QDs）的生物功能化的策略。该流动反应器能够在可控的表面功能化的情况下，可重现大量QD的合成。这些量子点充当荧光生物标志物，并作为糖基团向凝集素，受体和细胞呈递的结构支架。在发生相转移到水中之前，使用我们先前描述的点击化学方法将碳水化合物共价连接到两亲性聚（异戊二烯）-b-聚（乙二醇）二嵌段共聚物（PI-b-PEG）上。这些官能化的聚合物随后在连续流动系统中保留其独特的光学性质的情况下用于QD的封装。使用共聚焦显微镜对糖缀合的QD与人宫颈癌细胞系HeLa的结合进行了表征。取决于聚合物的端基（即：D-麦芽糖，D-葡萄糖，羧基和胺），可以控制和控制官能化QD的吸收。麦芽糖的功能化在低孵育时间和低浓度下产生了很高的摄取量。尽管已知血清会抑制人工纳米结构的细胞反应，但我们观察到在含血清的培养基中麦芽糖功能化的纳米容器的细胞摄取减少但显着。这种封装材料由于缺乏毒性以及非凡的稳定性，已经过测试，适用于体内肿瘤靶向。尽管此方法依赖于高度可重现的连续流系统，该系统可产生大量的定义明确，功能齐全，无毒且高度稳定的纳米颗粒，但该方法具有非凡的工业，生物学和医学意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号