Principles and Application of Intrinsic Forster Resonance Energy Transfer (iFRET) for Label-free Detection of Native Proteins

机译：无标记检测天然蛋白质的内在福斯特共振能量转移（iFRET）的原理和应用

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Tryptophan residues in proteins of interest were evaluated as FRET donors to facilitate the development of a label-free protein detection system, coined "intrinsic Foerster (or fluorescence) resonance energy transfer (iFRET)". iFRET fluorescence probes, composed of an efficient and tryptophan-specific FRET acceptor in addition to a target protein-specific ligand, selectively bind to the target proteins thereby enabling Foerster resonance energy transfer between the protein tryptophan residues and the iFRET probe. We have developed efficient iFRET acceptor fluorophores and a deep UV microscope, which were successfully applied to detect native target proteins in live cells.

机译：感兴趣的蛋白质中的色氨酸残留物被评估为FRET供体，以促进无标签蛋白质检测系统的开发，造成的“内在的FORENTER（或荧光）共振能量转移（IFRET）”。 IFRET荧光探针除了靶蛋白质特异性配体之外，由高效和色氨酸特异性的FRET受体组成，选择性地结合靶蛋白，从而使蛋白质色氨酸残基和IFRET探针之间的焦化谐振能量转移。我们已经开发了高效的IFRET受体荧光团和深紫色显微镜，其成功地应用于检测活细胞中的本地靶蛋白。

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《Conference on nano-bio sensing, imaging, and spectroscopy》|2015年|952306.1-952306.4|共4页
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Hyo Jin Kang; Ju Hwan Kim; Amar B. T. Ghisaidoobe; Sang J. Chung;
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tryptophan fluorescence; iFRET; label-free protein detection; cell imaging; deep UV biological microscopy;

机译：色氨酸荧光; iFRET;无标记蛋白质检测;细胞成像;深紫外生物显微镜;

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专利

1. Intrinsic Forster Resonance Energy Transfer Imaging Technique for Detection of Native Protein in Live Cells [J] . Lee Hanki, Kang Hyo Jin, Kim Ju Hwan, Bulletin of the Korean Chemical Society . 2016,第2期

机译：内在福斯特共振能量转移成像技术检测活细胞中的天然蛋白质
2. Induced Self-Assembly and Forster Resonance Energy Transfer Studies of Alkynylplatinum(ll) Terpyridine Complex Through Interaction With Water-Soluble Poly(phenylene ethynylene sulfonate) and the Proof-of-Principle Demonstration of this Two-Component Ensemble for Selective Label-Free Detection of Human Serum Albumin [J] . Clive Yik-Sham Chung, Vivian Wing-Wah Yam Journal of the American Chemical Society . 2011,第46期

机译：炔丙基铂（II）联吡啶配合物与水溶性聚苯撑乙炔磺酸盐的相互作用诱导的自组装和福斯特共振能量转移研究，以及该两组分组合的原理证明证明了该方法的选择性无标记检测人血清白蛋白
3. Homogeneous detection of caspase-3 using intrinsic fluorescence resonance energy transfer (iFRET) [J] . Kang Hyo Jin, Kim Ju Hwan, Chung Sang J. Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2015,第Null期

机译：使用固有荧光共振能量转移（iFRET）均质检测caspase-3
4. Principles and Application of Intrinsic Forster Resonance Energy Transfer (iFRET) for Label-free Detection of Native Proteins [C] . Hyo Jin Kang, Ju Hwan Kim, Amar B. T. Ghisaidoobe, Conference on nano-bio sensing, imaging, and spectroscopy . 2015

机译：内在饲料共振能量转移（IFRET）对天然蛋白质无标签检测的原理和应用
5. Single molecule and single nanoparticle spectroscopic studies using Forster resonance energy transfer and nanometal surface energy transfer in biological applications. [D] . Jaquins-Gerstl, Andrea. 2007

机译：在生物应用中使用Forster共振能量转移和纳米金属表面能转移的单分子和单纳米粒子光谱研究。
6. Intrinsic Tryptophan Fluorescence in the Detection and Analysis of Proteins: A Focus on Förster Resonance Energy Transfer Techniques [O] . Amar B. T. Ghisaidoobe, Sang J. Chung 2014

机译：蛋白质检测和分析中的固有色氨酸荧光：以福斯特共振能量转移技术为重点
7. Induced self-assembly and Förster Resonance Energy Transfer Studies of Alkynylplatinum(II) Terpyridine Complex through interaction with water-soluble Poly(phenylene ethynylene sulfonate) and the proof-of-principle demonstration of this two-component ensemble for selective label-free detection of Human Serum Albumin (HSA) [O] . CHUNG CYS, Yam VWW 2013

机译：通过与水溶性聚（亚苯基亚乙炔基磺酸盐）的相互作用诱导自组装和Förster共振能量转移研究炔基铂（II）三联吡啶络合物和这种双组分集合的原理证明，用于选择性无标记检测人血清白蛋白（Hsa）

Principles and Application of Intrinsic Forster Resonance Energy Transfer (iFRET) for Label-free Detection of Native Proteins

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