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An automated microfluidic assay for the detection of cancer biomarkers in serum using photonic crystal enhanced fluorescence

机译：使用光子晶体增强荧光检测血清中癌症生物标志物的自动化微流控分析

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An automated microfluidic photonic crystal enhanced fluorescence (PCEF) assay platform was recently reported for the multiplexed detection of cytokines spiked into phosphate buffered saline. In this work, we apply the aforementioned platform for cancer biomarker detection in human serum. Detection and control proteins were printed in a microarray format onto the surface of a photonic crystal designed to enhance the signal of fluorophores in the excitation and emission range of Cyanine 5 dye and similar alternatives. Fluorescence-linked immunosorbent assays (FLISAs) were performed, and a dose-response curve was obtained for human serum spiked with anti-E7 antibody, a known biomarker for human papillomavirus (HPV)-associated oropharyngeal cancer (OPC). Further, clinical samples of 20 OPC patients and 20 healthy controls were also tested using the PCEF platform, and the results were compared to those of the gold standard enzyme-linked immunosorbent assay (ELISA). Based on the results of a single trial of forty clinical samples, the sensitivity and specificity of the automated PCEF assay for anti-E7 is 75% and 65%, respectively, compared to 56% and 95% for ELISA.
机译：最近报道了一种自动化的微流控光子晶体增强荧光（PCEF）分析平台，用于对掺入磷酸盐缓冲液中的细胞因子进行多重检测。在这项工作中，我们将上述平台用于人类血清中癌症生物标志物的检测。将检测和对照蛋白以微阵列格式打印到光子晶体的表面上，该光子晶体旨在增强在花菁5染料和类似替代物的激发和发射范围内的荧光团信号。进行了荧光连锁免疫吸附测定（FLISA），并获得了掺有抗E7抗体的人血清的剂量反应曲线，抗E7抗体是人乳头瘤病毒（HPV）相关的口咽癌（OPC）的已知生物标志物。此外，还使用PCEF平台测试了20例OPC患者和20例健康对照的临床样品，并将结果与​​金标准酶联免疫吸附测定（ELISA）的结果进行了比较。根据一项针对40个临床样品的单项试验的结果，自动化PCEF分析对抗E7的敏感性和特异性分别为75％和65％，而ELISA分别为56％和95％。

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