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Application of Arrayed CRISPR/cas9 Screen and its Data Analysis: a Systematic Review

机译：阵列CRISPR / cas9筛选的应用及其数据分析：系统综述

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CRISPR has become a hot research area ever since its advent for its efficiency and specificity in editing almost every section of DNA sequences. Based on its function of gene perturbation, a variety of gene editing techniques have been developed to achieve different aims. The target locations of a DNA strain can be precisely broken and repaired, during which the genes can be removed, repaired, silenced, or activated. The high efficiency of CRISPR/Cas9 reagents preparation and the easiness of experiment conduction make it now a powerful tool of high-content screen. In a CRISPR mediated screen, the reagents targeting different genes can be transferred and cultured together as a pool or separately in arrayed microwells. With the development of new synthesis methods which deceases the cost of guide RNA library preparation, arrayed CRIPSR screen is becoming more feasible and is believed to play more important role in a varies of biological research areas for its gene editing specificity and variable phenotype measurements. So far, there are a number of experiments designed in arrayed strategy, however, to the best of our knowledge, there is not a review on these experiments and their data analysis methods. Thus, we designed this systematic review and performed meta-analysis for these studies. In this paper we review the research area, study design, method of transfer of guide RNAs, final measurement of readouts and data analysis methods.
机译：自从CRISPR技术在编辑DNA序列的几乎每个部分中的效率和特异性以来，它就成为一个热门的研究领域。基于其基因扰动的功能，已经开发了多种基因编辑技术来实现不同的目的。 DNA菌株的目标位置可以精确地断裂和修复，在此期间可以去除，修复，沉默或激活基因。 CRISPR / Cas9试剂制备的高效率和易于进行的实验使其成为高容量筛选的有力工具。在CRISPR介导的筛选中，靶向不同基因的试剂可以作为池一起转移或一起培养，也可以分别在阵列微孔中培养。随着降低指导RNA文库制备成本的新合成方法的发展，阵列CRIPSR筛选变得更加可行，并且据信其基因编辑特异性和可变表型测量在各种生物学研究领域中起着更重要的作用。到目前为止，有许多以阵列策略设计的实验，但是，据我们所知，还没有对这些实验及其数据分析方法进行评论。因此，我们设计了这项系统的综述，并对这些研究进行了荟萃分析。在本文中，我们回顾了研究领域，研究设计，指导RNA的转移方法，读数的最终测量和数据分析方法。

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