• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Photonics in Dermatology and Plastic Surgery >Effects of Timolol in Skin Tissue Repair: A Longitudinal and Multimodal In Vivo Imaging Study with Two-Photon Excitation Fluorescence and Second Harmonic Generation Microscopy
【24h】

Effects of Timolol in Skin Tissue Repair: A Longitudinal and Multimodal In Vivo Imaging Study with Two-Photon Excitation Fluorescence and Second Harmonic Generation Microscopy

机译：蒂莫尔在皮肤组织修复中的影响：具有双光子激发荧光和二谐波产生显微镜的体内成像研究中的纵向和多峰

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Topical timolol is a nonselective beta-adrenergic antagonist used to treat elevated intraopthalmic pressure, but it has also been shown to expedite time-to-wound closure in treating chronic, recalcitrant wounds and ulcers in off-label clinical studies. Current literature supports the theory of beta-adrenergic inhibition facilitating reepithelization through potentiating keratinocyte migration and angiogenesis. The purpose of our study is to use clinical imaging, two-photon excitation fluorescence (TPF) and second harmonic generation (SHG) microscopy to evaluate the temporal and molecular changes of the skin tissue during the wound healing process. In this study, timolol (n=11) and saline (n=11) treated Sprague-Dawley rats underwent a 5-millimeter dermal biopsy procedure to create an acute wound site on the dorsorostral back. Wound sites on both groups were imaged every other day until Day 14 and then every 4 days until Day 27 when the study concluded. During the study, timolol or saline were applied daily on the wound sites topically. On Day 0, 5, 14, and 27 the wound sites were harvested for histological review. Time-to-wound healing was assessed by comparing surface areas of the non-approximated wound margins. Qualitative assessment was used to determine microscopic collagenous and vascular changes on 3D reconstructions of TPF/SHG Z-stack images. Findings from clinical imaging revealed all rats exhibited approximation of wound borders by day 12. The control group demonstrated a more expedited rate of wound closure on day 4 (p = 0.026). TPF/SHG revealed increases in density and of collagen and vasculature in both groups over time. This may suggest factors other than the time-to-wound closure are important in the timolol-induced healing process described in prior research.
机译：局部蒂莫尔是一种非选择性β-肾上腺素能拮抗剂，用于治疗肝肿的脑内压力升高，但是还显示出加快缺血时间闭合，处理慢性，顽固的伤口和溃疡的临床研究中。目前的文献支持β-肾上腺素能抑制理论，促进通过增强角质形成细胞迁移和血管生成的再次入发。我们的研究目的是使用临床成像，双光子激发荧光（TPF）和二次谐波产生（SHG）显微镜，以评估伤口愈合过程中皮肤组织的时间和分子变化。在本研究中，蒂洛尔（n = 11）和盐水（n = 11）处理的Sprague-dawley大鼠经历了5毫米的皮肤活检程序，以在背体上产生急性缠绕位点。两组的伤口部位每隔一天成像，直到14天，然后每4天到第27天，当研究结束时。在研究期间，蒂莫尔或盐水局部每天在伤口部位施用。在第0,5,14和27天，收获伤口部位以进行组织学审查。通过比较非近似伤口边缘的表面积来评估缠绕的时间愈合。定性评估用于确定TPF / SHG Z堆​​叠图像的三维重建的微观胶虫和血管变化。临床成像的结果显示所有大鼠在第12天表现出伤口边界的近似。对照组在第4天（P = 0.026）上表现出更快速的伤口闭合速率（P = 0.026）。 TPF / SHG随着时间的推移，在两组中揭示了密度和胶囊和血管系统的增加。这可能表明除了缠绕的时间闭合以外的因素在先前研究中描述的氏炎诱导的愈合过程中是重要的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号