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首页> 外文会议>Conference on Clinical and Translational Neurophotonics >Use of a Conformational Switching Aptamer for Rapid and Specific Ex Vivo Identification of Central Nervous System Lymphoma in a Xenograft Model
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Use of a Conformational Switching Aptamer for Rapid and Specific Ex Vivo Identification of Central Nervous System Lymphoma in a Xenograft Model

机译：构象转换适体用于异种移植模型中的中枢神经系统淋巴瘤的快速和特异体内鉴定的用途

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Improved tools for providing specific intraoperative diagnoses could improve patient care. In neurosurgery, intraoperatively differentiating non-operative lesions can be challenging, often necessitating immunohistochemical (IHC) procedures which require up to 24-48 hours. Here, we evaluate the feasibility of generating rapid ex vivo specific labeling using a novel lymphoma-specific fluorescent switchable aptamer. Our B-cell lymphoma-specific switchable aptamer produced only low-level fluorescence in its unbound conformation and generated an 8-fold increase in fluorescence once bound to its target on CD20-positive lymphoma cells. The aptamer demonstrated strong binding to B-cell lymphoma cells within 10 minutes of incubation. We applied the switchable aptamer to ex vivo xenograft tissue harboring B-cell lymphoma and astrocytoma, and within one hour specific visual identification of lymphoma was routinely possible. In this proof-of-concept study in human cell culture and orthotopic xenografts, we conclude that a fluorescent switchable aptamer can provide rapid and specific labeling of B-cell lymphoma, and that developing aptamer-based labeling approaches could simplify tissue staining and drastically reduce time to histopathological diagnoses compared with IHC-based methods. We propose that switchable aptamers could enhance expeditious, accurate intraoperative decision-making.
机译：用于提供特定术中诊断的改进工具可以改善患者护理。在神经外科术中，术中分化的非手术性病变可能是具有挑战性的，通常需要预达24-48小时的免疫组织化学（IHC）程序。在这里，我们评估使用新型淋巴瘤特异性荧光可切换适体产生快速exvivo特异标记的可行性。我们的B细胞淋巴瘤特异性可切换适体仅在其未结合构象中产生低水平的荧光，并产生荧光的8倍，一旦与其对CD20阳性淋巴瘤细胞结合的靶标。适体在孵育10分钟内表现出对B细胞淋巴瘤细胞的强烈结合。我们将可切换适体的可切换适体涂覆到含B细胞淋巴瘤和星形细胞瘤的前体内异种移植组织，并且在一小时内常规可视地鉴定淋巴瘤。在这种概念验证中，在人细胞培养和原位异种移植物中，我们得出结论，荧光可切换适体可以提供B细胞淋巴瘤的快速且特异性标记，并且开发基于适体的标记方法可以简化组织染色并大大减少与基于IHC的方法相比，组织病理学诊断的时间。我们建议可切换的适体可以增强迅速，准确的术中决策。

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