• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Advanced Computing and Applications >Trimer Distribution Patterns in the Protein Sense and Antisense Sequences on the two Replichores of the Burkholderia Lata Chromosomes
【24h】

Trimer Distribution Patterns in the Protein Sense and Antisense Sequences on the two Replichores of the Burkholderia Lata Chromosomes

机译：Burkholderia Lata染色体两个复制体上蛋白质有义和反义序列中的三聚体分布模式

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Burkholderia lata is a bacterium whose cell has three circular chromosomes, which are different in both size and nucleotide sequence. Each chromosome has a replication origin and a replication terminus, which divide the chromosome into two halves, forming the two replichores during the process of DNA replication. The complete bacterial chromosomal sequence is made of protein sense and antisense sequences, intergenic sequences, and sequences that encode for ribosomal RNAs and transfer RNAs. The natural nucleotide distribution in the bacterial chromosome has not much been known, while it is vital to the DNA replication rate, the cell division process and the chromosomal stability, which can in turn exert effects on the success of the genetic experiments manipulating the bacterial chromosomes. We used computer, and randomly chose a pair of trimer/trimer's reverse complement, GCG/CGC, among 32 possible trimer/trimer's reverse complement pairs, to perform our investigation on their distribution in the protein sense and antisense sequences of each of the B. lata chromosomes. We found that, although the chromosomes were different in size and nucleotide sequence, the distribution patterns of the GCG and CGC were the same in all these chromosomes, in that the frequencies of certain distances (frequencies of D) between the adjacent GCGs in the sense sequences (S) on one replichore (R1) of a chromosome were almost similar to those between the adjacent CGCs in the antisense sequences (AS) on the other replichore (R2) of that chromosome; also, the frequencies of D between the adjacent GCGs in the AS on R1 were almost similar to those between the adjacent CGCs in the S on R2, and vice versa. This finding provides key information of the natural nucleotide distribution in the bacterial chromosomes, which can help in experimental design for success in manipulation of the bacterial chromosomal sequences or in evolutionary analysis of protein sequences.
机译：伯克霍尔德氏菌是一种细菌，其细胞具有三个环形染色体，其大小和核苷酸序列均不同。每个染色体都有一个复制起点和一个复制末端，将染色体分成两半，在DNA复制过程中形成两个复制体。完整的细菌染色体序列由蛋白质有义和反义序列，基因间序列以及编码核糖体RNA和转移RNA的序列组成。细菌染色体中的天然核苷酸分布尚不为人所知，尽管它对DNA复制速率，细胞分裂过程和染色体稳定性至关重要，而这反过来又会对操纵细菌染色体的基因实验的成功产生影响。 。我们使用计算机，在32种可能的三聚体/三聚体反向互补对中随机选择一对三聚体/三聚体反向互补序列GCG / CGC，以研究它们在每个B的蛋白质有义和反义序列中的分布。拉塔染色体。我们发现，尽管染色体的大小和核苷酸序列不同，但是GCG和CGC的分布方式在所有这些染色体中都是相同的，因为相邻GCG之间存在一定距离（D频率）的频率染色体一个复制体（R1）上的序列（S）与该染色体另一复制体（R2）上反义序列（AS）中相邻CGC之间的序列几乎相似;同样，R1上AS的相邻GCG之间的D频率几乎与R2上S上的相邻CGC之间的D频率相似，反之亦然。该发现提供了细菌染色体中天然核苷酸分布的关键信息，可帮助成功进行细菌染色体序列操作或蛋白质序列进化分析的实验设计。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号