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首页> 外文会议>Water and environment technology conference >DNA-Based Discrimination and Population Genetics of the Coconut Scale Insect Aspidiotus Rigidus Reyne in the Philippines
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DNA-Based Discrimination and Population Genetics of the Coconut Scale Insect Aspidiotus Rigidus Reyne in the Philippines

机译：基于DNA的菲律宾椰子皮鳞茎天蛾（Repidustus Rigidus Reyne）的鉴别和种群遗传学

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摘要

The Philippines faced a devastating coconut scale insect (CSI) outbreak damaging 2.7 million coconut palms in the Calabarzon region from 2009-2014, and in Basilan and Zamboanga until recently. Initially, the damage was thought to be caused by Aspidiotus destructor Signoret which is native to the Philippines but further morphological analysis pointed A. rigidus as the real culprit. These two species are difficult to identify morphologically, but identification is possible based on ecological features. This study aims to provide molecular genetic evidence to support the use of these ecological characteristics to differentiate the two species from one another. We also aim to estimate the migration pattern of A. rigidus from the previously reported infestation in the Philippines. We extracted DNA from a total of 595 individuals of 3 species of CSI (A. rigidus, A. destructor and A. excisus), and sequenced the mitochondrial cytochrome oxidase subunit I (COI) gene region and the nucleotide elongation factor 1α (EF-1α) gene region. Sequence data from both gene regions distinguished the A. rigidus against the A. destructor, and provided new knowledge that the source of the group of A. rigidus is different in north and south of the Philippines.
机译：从2009年至2014年，菲律宾在Calabarzon地区以及直到最近的Basilan和Zamboanga遭遇毁灭性的椰子鳞虫（CSI）爆发，造成270万株椰子树受损。最初，这种破坏被认为是由原产于菲律宾的Aspidiotus破坏者Signoret引起的，但进一步的形态学分析表明，僵硬曲霉是真正的元凶。这两个物种很难从形态上进行识别，但是可以根据生态特征进行识别。这项研究旨在提供分子遗传学证据，以支持利用这些生态特征来区分这两个物种。我们还旨在从先前报道的菲律宾侵染中估计僵硬曲霉的迁徙模式。我们从CSI的3种物种的595个个体中提取DNA，对线粒体细胞色素氧化酶亚基I（COI）基因区域和核苷酸延伸因子1α（EF- 1α）基因区域。来自两个基因区域的序列数据区分了僵硬曲霉和毁灭曲霉，并提供了新的知识，即僵硬曲霉群的来源在菲律宾的北部和南部是不同的。

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